Expression of vascular endothelial cell growth factor and regeneration of hepatic endothelial cells during liver regeneration.

大鼠部分肝切除后，残肝在一周内迅速再生。为了评估血管生成生长因子在肝内皮细胞再生中的作用，我测定了血管内皮细胞生长因子（VEGF- a和VEGF- b）和碱性成纤维细胞生长因子（beGf）在再生肝脏和其他器官中的表达，因为低分子量VEGF （VEGF 121）可以进入血液并可能参与再生肝脏的血管内皮细胞。VEGF-A表达在肝部分切除后立即升高，并维持高水平表达。bFGF表达有升高趋势，VEGF-B表达无变化。在再生肝脏中，VEGF-A和VEGF-B的剪接变体未见变化。肺和心脏持续高水平表达VEGF- a，前者表达VEGF，而后者不表达VEGF 121。在肝脏再生过程中，VEGF-A在两器官的表达均无明显变化。脾脏和肾脏中VEGF-A表达几乎翻倍，脾脏中检测到VEGFl2 I。VEGF-B在肺、心、脾、小肠的表达均升高，而在肝、肾的表达无明显变化。这些结果表明，再生肝脏中VEGF-A和bFGF的表达可能促进了非实质细胞的再生，肺和脾中产生的VEGFl2I和肺、心、脾和小肠中产生的VEGF-B也可能参与了再生。这些因子表达增加的机制尚不清楚。然而，我们发现VEGF-A和bFGF在肌肉脂肪组织中的表达受交感神经的控制。还应注意的是，交感神经系统在肝脏再生过程中被激活。因此，VEGF-A、VEGF-B和bFGF的表达可能受肌肉脂肪组织等交感神经系统的调控。少

After partial hepatectomy in the rat, the remnant liver rapidly regenerates within a week. To evaluate the involvement of angiogenic growth factor in regeneration of hepatic endothelial cells, I determined the expression of vascular endothelial cell growth factor (VEGF-A and VEGF-B) and basic fibroblast growth factor (beGf) in the regenerating liver and other organs since low molecular weight VEGF (VEGF 121) could enter the blood and might be involved in vascular endothelial cells in the regenerating liver, In the liver, VEGF-A expression was increased immediately after partial hepatectomy, and sustained high level of the expression. The expression of bFGF was tend to increase, but the expression of VEGF-B was not changed. No change was observed in splicing variants of VEGF-A and VEGF-B in the regenerating liver. Lung and heart persistently expressed VEGF-A at high levels, and the former, but not the latter, expressed VEGF 121. During the regeneration of liver, no apparent change was s … More een in the expression of VEGF-A in the both organs. The VEGF-A expression in spleen and kidney was almost doubled, and VEGFl2 I was detected in spleen. The expression of VEGF-B was increased in lung, heart, spleen and small intestine, although it was not changed in liver and kidney. These results suggest that hepatic expression of VEGF-A and bFGF in the regenerating liver might contribute the regeneration of non-parenchymal cells, and that VEGFl2I produced in lung and spleen and VEGF-B from lung, heart, spleen and small intestine might also be involved in the regeneration. The mechanism by which expression of these factors increases is still obscure. However, we found the expression of VEGF-A and bFGF in brawn adipose tissue was controlled by the sympathetic nerve. It should be also noted that the sympathetic nerve systems are activated during the liver regeneration. Therefore, the expression of VEGF-A, VEGF-B and bFGF might be regulated by the sympathetic nerve systems like brawn adipose tissue. Less