Pilot Project Core

试点项目总结 华盛顿大学阿片成瘾研究卓越中心将建立一个试点项目 旨在支持每年2个新项目的计划。建议的试验计划将每年向 NIDA P30调查员。这些建议将由外部咨询委员会根据 一套简单的标准：将优先考虑以下建议：1)解决阿片类药物使用障碍的一个方面，2) 支持年轻研究人员，3)利用影像和分子遗传学资源，4)强调 创新/高风险。对补充现有资金的提案将给予较低的优先级。我们期待着 一些试点项目提案将来自我们的NIDA-T32 DA 007278支持的学员。提供 对已经获得T32或个人NRSA奖项津贴支持的研究员的研究支持将 利用试点项目资金，加强我们的培训计划。根据这些标准，排名前两位的项目 将被选中，申请者将被要求将他们的大纲扩展到5页长的描述，其中包括 具体目标、研究计划和交付成果。这些建议将再次由委员会成员审查 将提供建设性意见的外部咨询委员会。一旦纳入这些建议， 在提供资金之前，2项提案将被提交给NIDA计划进行额外考虑。基于这些 根据标准，在这份初次提交的文件中选择了两项提案。试验计划1描述建议进行的研究 在苏珊·弗格森博士的实验室工作的研究生蒂姆·奥尼尔先生(奥尼尔先生有津贴支持 来自NIDA F31 NRSA)。该项目将使用体内钙成像来分析反应和兴奋性。 海洛因自我给药过程中PFC神经元的变化。试点项目2描述了Dr。 安东尼·亚伯拉罕，查尔斯·查夫金博士实验室的博士后研究员。(亚伯拉罕博士已经 由华盛顿大学的NIDA T32支持)。他的项目将评估新型活动传感器，旨在表征 吗啡戒断诱导强啡肽释放过程中PFC神经元Kappa阿片受体的激活。 研究表明，应激诱导的强啡肽释放能促进焦虑不安的反应，从而引发渴望和 复发，但作用机制和作用地点尚未确定。未来的试点项目将是 审查后每年启动一次。

Pilot Project Summary The University of Washington Center of Excellence in Opioid Addiction Research would establish a pilot project program designed to support 2 new projects per year. Proposed pilot projects will be solicited annually from the NIDA P30 Investigators. These proposals would be ranked by the External Advisory Committee based on a simple set of criteria: Priority will be given to proposals that 1) address an aspect of opioid use disorder, 2) support young investigators, 3) utilize the Imaging and Molecular Genetics resources, and 4) emphasize innovation/high risk. Low priority will be given to proposals that supplement existing funding. We expect that some of the pilot project proposals will come from trainees supported by our NIDA-T32 DA 007278. Providing research support to fellows already having stipend support from the T32 or an individual NRSA award will leverage the Pilot Project funds and strengthen our training program. Based on these criteria, the top 2 projects will be selected and the applicants will be asked to expand their outlines to a 5-page description that includes specific aims, research plan and deliverables. These proposals will again be reviewed by the members of the External Advisory Committee who will provide constructive input. Once these suggestions are incorporated, the 2 proposals will be forwarded to NIDA Program for additional consideration prior to funding. Based on these criteria, two proposals have been selected in this initial submission. Pilot Project 1 describes studies proposed by Mr. Tim O'Neal, a graduate student working in Dr. Susan Ferguson's lab (Mr. O'Neal has stipend support from a NIDA F31 NRSA). This project would use in vivo calcium imaging to analyze responses and excitability changes in PFC neurons during heroin self-administration. Pilot Project 2 describes studies proposed by Dr. Antony Abraham, a postdoctoral fellow working in Dr. Charles Chavkin's lab. (Dr. Abraham has been supported by the UW's NIDA T32). His project would evaluate novel activity sensors designed to characterize kappa opioid receptor activation in PFC neurons during morphine-withdrawal induced dynorphin release. Stress-induced dynorphin release has been shown to promote a dysphoric response that triggers craving and relapse, but the mechanisms and sites of action have not yet been characterized. Future Pilot Projects will be initiated annually after review.