GENETIC MANAGEMENT OF CHIMPANZEE BREEDING AND RESEARCH

该提案的总体目标是为黑猩猩育种服务， 研究计划（CBRP），提供全面的遗传管理。 为此，我们将证实所有CBRP黑猩猩的亲子关系， 在5个殖民地的每个殖民地都有专门的繁殖队列， 量化遗传变异的水平。 这些数据将被用作 为合理的育种计划奠定基础。 我们将使用DNA指纹 以确定亲子关系并监测血统。 我们还将类型 多态性遗传标记，包括同工酶，转铁蛋白，RFLP， VNTR和二核苷酸重复序列。 标记数据将用于 计算表征每个群体的群体遗传参数， 可以估计遗传多样性。 基因数据将 为将非CBRP男性引入 现有的殖民地-作为育种者，以提高基因库。 他们还将 提供选择特定后代的基本原理 在专门的育种队列中包括过多的父母。 一个 该项目提供的服务的重要成果是， 确保黑猩猩种群的遗传多样性 从而确保它们继续用于生物医学 目的

The overall goal of this proposal is to serve the Chimpanzee Breeding and Research Program (CBRP) by providing comprehensive genetic management. To this end, we will corroborate parentage of all CBRP chimpanzees in the dedicated breeding cohorts at each of the 5 colonies and empirically quantify levels of genetic variability. These data will be used as a basis for a rational breeding program. We will use, DNA fingerprinting to ascertain parentage and to monitor the pedigrees. We will also type for polymorphic genetic markers, including isozymes, transferrins, RFLPs, VNTRs, and dinucleotide repeats. The marker data will be used to calculate population genetic parameters that characterize each colony and allow for an estimate of genetic diversity. The genetic data will provide guidelines for the introduction of nonCBRP males into the existing colonies-as breeders to enhance the gene pool. They will also provide the rationale for selecting specific offspring of over-represented parents to include in the dedicated breeding cohort. An important result of the service provided by this project will be to assure the maintenance of a genetically diverse population of chimpanzees in captivity and thus assure their continued availability for biomedical purposes.