RESOURCE FOR CONSTRUCTION OF 2D GEL PROTEIN DATABASES

冷泉探索号资源设施的长期目标 港湾实验室将开发2D凝胶蛋白质数据库作为强大工具 用于生物研究。具体地说，该设施寻求1)进一步 发展二维蛋白质分析的计算机技术 凝胶，2)在三个重要研究中建立蛋白质模型数据库 以及3)以其他形式传播这些数据库 研究人员可以在个人电脑上使用。 任务资源实际上是两种资源合二为一。在计算机方面， 软件开发人员将寻求使2D凝胶图像分析几乎 完全自动化，他们将寻求提高 科学家通过改进用户界面来建立蛋白质数据库 数据库管理工具。在实验室方面， Quest设备将执行核心和协作实验，以建立 蛋白质数据库，并识别大量检测到的蛋白质 在二维凝胶上。 将为以下研究领域开发数据库：1)细胞- 哺乳动物细胞的增殖，2)核基因调节蛋白，以及 3)酵母细胞与分子生物学。每个蛋白质数据库都建立在 之前在Quest设施和科学研究方面的经验 核心员工和协作型员工。 Quest实验室将包括一个蛋白质化学设施。 身份证明。微测序将被常规用于识别 二维凝胶中的蛋白质。抗体将被收集并使用 以确定当前感兴趣的蛋白质。多肽图谱和氨基酸 组合物将提供与蛋白质相关的额外信息 来自不同物种和不同实验室的地图。 QUEST蛋白质数据库将分发给其他研究人员 用于Macintosh计算机的CD-ROM盘。凝胶图像，定量 将为每个项目提供数据、注释和实验室记录 做实验。数据将与HyperCard和许多 用于统计分析和数据展示的软件包，包括 现已在Macintosh上提供。 作为一种研究正常和疾病相关蛋白质图谱的技术 对于细胞和体液，2D凝胶蛋白质数据库将同时拥有这两个 对健康问题的直接和长期影响。已标记 已经发现了白血病等疾病的蛋白质。在漫长的岁月里 术语，自动化2D凝胶分析，结合广泛的蛋白质数据库， 不仅应该根据标记蛋白，而且应该根据整个 蛋白质谱包括遗传多态、调节改变 蛋白质(癌基因产物)和对外部因素的反应(病毒、 压力、毒品)。

The long-term objective of the QUEST resource facility at the Cold Spring Harbor Laboratory is to develop 2D gel protein databases as powerful tools for biological research. Specifically, this facility seeks 1) to further develop the computer technology for analysis of two-dimensional protein gels, 2) to build model protein databases in three important research areas, and 3) to disseminate these databases in a form that other researchers can use on personal computers. The QUEST resource is really two resources in one. On the computer side, the software developers will seek to make 2D gel image analysis almost totally automatic, and they will seek to improve the productivity of the scientists who build protein databases by improving the user interface and the database management tools. On the laboratory side, the members of the QUEST facility will perform core and collaborative experiments to build protein databases and to identify large numbers of the proteins detected on two dimensional gels. Databases will be developed for the following research areas: 1) cell- proliferation in mammalian cells, 2) nuclear gene-regulatory proteins, and 3) yeast cell and molecular biology. Each protein database builds on previous experience at the QUEST facility and on the scientific research of core and collaborative workers. The QUEST laboratory will include a protein chemistry facility for protein identifications. Microsequencing will be used routinely to identify proteins from two-dimensional gels. Antibodies will be collected and used to identify proteins of current interest. Peptide profiles and amino acid compositions will provide additional information to relate the protein maps from different species and from different laboratories. The QUEST protein databases will be disseminated to other researchers on CD-ROM disks for use on Macintosh computers. Gel images, quantitative data, annotations, and laboratory records will be provided for each experiment. The data will be compatible with Hypercard and numerous software packages for statistical analysis and data presentation that are now available on the Macintosh. As a technology to study the normal and disease-related protein profiles of cells and body fluids, 2D gel protein databases will have both immediate and long-term impact on health problems. Already marker proteins for diseases such as leukemias have been found. In the long term, automated 2D gel analysis, coupled with extensive protein databases, should give diagnoses based not just on marker proteins but on the entire protein profile including genetic polymorphisms, altered regulatory proteins (oncogene products), and responses to external agents (viruses, stress, drugs).