METABOLIC IMAGING OF LIVERS AWAITING TRANSPLANT

描述（改编自申请人的摘要）：长期目标 本研究旨在开发无创光学仪器和方法， 监测代谢状态并评估功能完整性 在移植前冷保存和冷灌注的肝脏。本研究 是基于一种假设，即在低温储存的 肝脏，可以检测到氧化代谢功能障碍的区域 使用分光光度法非侵入性地进行。第一阶段a 组装了计算机控制的成像系统，并成功地 开发用于获取和分析细胞色素氧化还原和NADH荧光 来自冷储存和冷灌注的大鼠肝脏的图像。比较 正常和化学损伤的肝脏产生了有希望的指标， 组织损伤，将在第二阶段进行研究。二期 开发将集中在准备和测试成像系统， 用于临床研究。与该司的合作伙伴 华盛顿大学医学中心的移植将测试 一家研究医院的二期原型一种非侵入性的 等待移植的肝脏中的氧化代谢将同时具有 临床和研究应用。检测肝脏的能力 移植前的功能障碍将改善移植计划， 建立肝脏活力的能力将有助于研究 增加器官储存时间

DESCRIPTION (Adapted from applicant's abstract): The long term goal of this research is to develop noninvasive optical instruments and methods to monitor the metabolic state and assess the functional integrity of cold-stored and cold-perfused livers prior to transplant. This research is based on the working hypothesis that in hypothermically-stored livers, areas with dysfunctional oxidative metabolism can be detected noninvasively using spectrophotometric methods. During Phase I a computer-controlled imaging system was assembled and methods successfully developed to obtain and analyze cytochrome redox and NADH fluorescence images from cold-stored and cold- perfused rat livers. Comparison of normal and chemically-damaged livers yielded promising indicators of tissue damage which will be explored during Phase II. Phase II development will focus on preparing and testing the imaging system for use in clinical research. A subcontract with the Division of Transplantation at the University of Washington Medical Center will test a Phase II prototype at a research hospital. A noninvasive monitor of oxidative metabolism in livers awaiting transplant will have both clinical and research applications. The ability to detect liver dysfunction prior to transplantation will improve transplant planning, and the ability to establish liver viability will aid research directed towards increasing organ storage time.