STUDIES ON TOXIC POTENTIAL OF RETROVIRAL VECTORS

慢性前期研究将确定什么暴露方案最大限度地提高了 经腹膜腔注射沙门氏菌感染媒介的可能性 丙型肝炎小鼠白血病细胞含LacZ基因载体的构建 病毒。这些研究还将评估监测方法，以 在选定的组织中存在载体衍生的DNA。对于慢性病前期患者 研究表明，新生和断奶的F344大鼠和B6C3F1小鼠都将 使用。 这项慢性研究将评估相关的致癌潜力 将逆转录病毒载体随机插入器官的细胞DNA 以及F344大鼠和B6C3F1小鼠的组织。根据调查结果 慢性前期研究，慢性研究将涉及新生儿或 并将包括被确定为最多的治疗方案 有效。

The prechronic studies will determine what exposure regimen maximizes the probability of vector infection following intraperitoneal injection with a LacZ containing vector constructed from the Maloney Murine Leukemia Virus. These studies will also evaluate methods of monitoring for the presence of vector-derived DNA in selected tissues. For the prechronic studies, both neonate and weanling F344 rats and B6C3Fl mice will be used. The chronic study will evaluate the carcinogenic potential associated with random insertion of a retroviral vector into cellular DNA of organs and tissues of F344 rats and B6C3Fl mice. Based on results of the prechronic studies, the chronic study will involve either neonates or weanlings and will include the treatment regimen determined to be most effective.