METHODS FOR DETECTION OF BK VIRUS IN ALLOGENEIC BONE MARROW TRANSPLANT

人多瘤病毒BK病毒感染高比例的 一般人群，并在初次接种后仍潜伏在肾脏中 感染当T细胞功能受损时，可以发生再激活。 有缺陷的（例如，在骨髓或器官移植的接受者中）。 BK病毒尿也被认为是出血性 骨髓移植患者的膀胱炎。快速检测 活跃的BK病毒可能有助于医疗管理这些 患者 开发了BK病毒的72小时壳瓶培养试验， 评价BK病毒在骨中的发病率及意义 骨髓移植患者人群。聚合酶的开发 用于检测BK病毒的链式反应（PCR）分析正在进行中， 并将进行临床比较，以确定是否 脱落细胞壳瓶培养、PCR或细胞学检查 尿上皮细胞是最敏感和快速的方法， 检测BK病毒

The human polyomavirus BK virus infects a high proportion of the general population and remains latent in the kidney after primary infection. Reactivation can occur when T cell functions are deficient (e.g., in recipients of bone marrow or organ transplants). BK viruria has also been implicated as a cause of hemorrhagic cystitis in bone marrow transplant patients. Rapid detection of active BK virus may help in the medical management of these patients. A 72-hour shell vial culture assay for BK virus was developed to evaluate the incidence and significance of BK virus in the bone marrow transplant patient population. Development of a polymerase chain reaction (PCR) assay for detection of BK virus is under way, and a clinical comparison will be performed to determine whether shell vial culture, PCR, or cytological examination of exfoliated urinary epithelial cells is the most sensitive and rapid method for detecting BK virus.