Ultrafast magic angle spinning solid-state nuclear magnetic resonance capability

该项目旨在扩展现有的核磁共振(核磁共振)光谱仪，用于固态蛋白质的结构研究。许多蛋白质，如淀粉样蛋白和柔性蛋白质，无法用X射线结晶学、溶液核磁共振光谱或冷冻电子显微镜进行研究，因为它们不能结晶或不能充分溶解，或者结构上太不均匀。该项目将现有800 MHz核磁共振光谱仪的能力扩展到固态核磁共振。通过提供超快的魔角旋转速度，该系统旨在为蛋白质系统提供大大增强的灵敏度和多维核磁共振谱，而这些核磁共振谱以前不适合通过核磁共振谱或其他技术进行结构分析。这将在生物技术和生物医学中有重要的应用。

This project aims to extend an existing nuclear magnetic resonance (NMR) spectrometer for structural investigations of proteins in the solid state. Many proteins, such as amyloids and flexible proteins, cannot be studied by X-ray crystallography, solution NMR spectroscopy or cryoelectron microscopy, because they cannot be crystallised or are not sufficiently soluble, or are structurally too heterogeneous. This project will extend the capability of an existing 800 MHz NMR spectrometer to solid-state NMR. By offering ultrafast magic angle spinning speeds, the system aims to afford greatly enhanced sensitivity and multidimensional NMR spectra of protein systems not previously amenable to structural analysis by NMR spectroscopy or other techniques. This will have important applications in biotechnology and biomedicine.