FOLLICULAR GROWTH AND ATRESIA

本研究的长期目标是了解 卵巢功能在组织水平，通过定义的机制， 允许一个卵泡完成卵泡发育的过程， 排卵，而降级别人闭锁，发展之前， 完成 本提案中的实验重点关注FSH在以下方面的作用： 选择过程。 该项目的具体目标是： 1)为了明确FSH升高募集卵泡的机制， 监测颗粒细胞增殖率、闭锁发生率， 操纵内源性FSH后细胞分化的证据 浓度的 2)描述FSH与早期胃窦炎的反馈关系， 卵泡在发情期的选择过程中，通过a）改变卵泡的数量， 早期有腔卵泡或循环中FSH的浓度 发情期，并确定这些干预措施是否可以防止 发情后期的完整大鼠中的闭锁卵泡，和B）比较 完整大鼠的选择过程与对数期半去势大鼠的选择过程相同， 在没有闭锁的情况下进行选择。 3)为了验证地下室成分的变化 膜参与闭锁的机制， 免疫组织化学技术检测分子变化， 选择早期胃窦时基膜的组成 发情期的卵泡 4)为了确定月经期黄体的机制， 周期抑制恒河猴卵泡发育，通过监测 颗粒细胞增殖率和闭锁发生率 在不同大小的卵泡中进行黄体切除术后。

The long-term objective of this research is to understand the regulation of ovarian function at the tissue level, by defining the mechanisms which permit one follicle to complete the process of follicular development and ovulate, while relegating others to atresia, before development is completed. The experiments in this proposal focus on the role of FSH in the selection process. The specific aims of this project are: 1) To define the mechanism by which elevated FSH recruits follicles by monitoring rates of granulosa cell proliferation, incidence of atresia, and evidence of cell differentiation following manipulation of endogenous FSH concentrations. 2) To characterize the feedback relationship between FSH and early antral follicles during the selection process on estrus by a) altering the number of early antral follicles or the concentration of FSH in the circulation on estrus and determining if these interventions prevent the appearance of atretic follicles in the intact rat on metestrus, and b) comparing the selection process in intact rats with that in log-term hemicastrate rats, where selection occurs without atresia. 3) To test the hypothesis that changes in the composition of the basement membrane are involved in the mechanisms of atresia by using immunohistochemical techniques to examine changes in the molecular composition of the basal lamina during the selection of early antral follicles on estrus. 4) To determine the mechanism by which the corpus luteum of the mentrual cycle inhibits follicular development in the rhesus monkey, by monitoring proliferation rates of granulosa cells and the incidence of atresia following luteectomy in different size classes of follicles.