NUCLEAR SUBSTRUCTURE IN RNA PROCESSING AND TRANSPORT

控制信息分子（mRNA）从细胞核流出 是细胞和分子生物学的一个重要领域 我们对此知之甚少 最近的证据表明 在细胞核中有一个高度组织化的系统， 以及前体向mRNA的转运。 大量的信息关于 这个系统可以通过研究原子核的结构 其中mRNA前体被包装的颗粒，hnRNP。 可惜 不可能纯化和分离含有特异性hnRNA的hnRNP， 研究其结构。 我们建议开发一个系统， 含有研究者选择的特定转录物的hnRNP可以是 以足够的量和纯度分离以研究它们的结构。 这 将通过将感兴趣的基因插入非缺陷的 在有效启动子控制下的腺病毒5表达载体。 使用该系统，我们将确定多肽和snRNP组成 hnRNP含有人组蛋白H3，鸡α-肌动蛋白和小鼠 α-珠蛋白转录物（有和没有它们的天然干预） 序列）。 我们还将启动α-珠蛋白和组蛋白的研究 hnRNP的最终目标是确定 和平。

The control of the flow of informational molecules (mRNAs) from the nucleus to the cytoplasm is an important area of cellular and molecular biology about which very little is known. Recent evidence suggests the existence of a highly organized system in the nucleus which regulates the processing and transport of precursors to mRNA. A great deal of information about this system can be learned from studying the structure of the nuclear particles in which mRNA precursors are packaged, hnRNP. Unfortunately, it is not possible to purify and isolate hnRNP containing specific hnRNAs in order to study their structure. We propose to develop a system with which hnRNP containing specific transcripts of the investigator's choice can be isolated in sufficient quantity and purity to study their structure. This will be accomplished by inserting genes of interest into a non-defective adenovirus 5 expression vector under the control of an efficient promoter. Using this system, we will determine the polypeptide and snRNP composition of hnRNPs containing human histone H3, chicken Alpha-actin and mouse Alpha-globin transcripts (with and without their natural intervening sequences). We will also initiate studies on Alpha-globin and histone hnRNP with the ultimate goal of determining the fine structure of the pacticles.