GENETICS OF T-LYMPHOCYTE ANTIGEN RECEPTORS

我们计划研究T淋巴细胞识别抗原的遗传学。 T细胞杂交瘤、抗受体抗体和受体DNA探针 基因。我们的一般方法是在杂交瘤中检测V基因 已知的特异性和相关的特异性，以便相互关联 具有Ag/MHC识别功能的受体结构。 具体项目包括绘制6号染色体上的Beta基因座， 识别常规抗原杂交瘤中V基因的检测 (卵清蛋白)或同种异体抗原(I-Ab)，或自身抗原(DD)。我们会计划 对一个阿尔法链多肽进行测序，以便克隆其中的基因 轨迹。最后，我们建议检查受体的表达。 发育中的胸腺。最终，我们希望更好地理解Ag/MHC是如何 识别是在基因组中编码的。

We plan to study the genetics of antigen recognition in T lymphocytes using T cell hybridomas, anti-receptor antibodies and DNA probes for receptor genes. Our general approach is to examine the V genes in hybridomas of known specificity and those of related specificity in order to correlate receptor structure with Ag/MHC recognition. Specific projects include mapping the beta locus on chromosome 6, examination of V genes in hybridomas which recognize a conventional antigen (ovalbumin) or an alloantigen (I-Ab), or a self-antigen (Dd). We will plan to sequence an alpha chain polypeptide in order to clone genes in this locus. Finally, we propose to examine receptor expression in the developing thymus. Ultimately, we hope to better understand how Ag/MHC recognition is encoded in the genome.