Nuclear plasticity during neutrophil migration and function

该项目旨在发现细胞核形状如何影响中性粒细胞功能。细胞迁移需要整体的细胞可塑性和细胞核等内部结构的可塑性。中性粒细胞是最常见的细胞类型之一，它有一个特殊的分叶状细胞核，被认为有助于其移动和功能。利用同时显示细胞核和细胞质的斑马鱼报告线，该项目将展示体内中性粒细胞迁移和功能过程中中性粒细胞核的动态可塑性。本项目试图使用格子光片显微镜的时空分辨率来进一步研究这一点，并探索其对中性粒细胞功能的影响。该项目寻求建立形态和机械原理，不仅适用于中性粒细胞，而且适用于所有类型的迁移细胞。

This project aims to discover how nuclear shape affects neutrophil function. Cell migration needs overall cellular plasticity and plasticity of internal structures such as the nucleus. The neutrophil, one of the most peripatetic cell types, has a specialised lobulated nucleus, thought to facilitate its mobility and function. Using zebrafish reporter lines that concurrently display the nucleus and cytoplasm, this project will display the dynamic plasticity of neutrophil nuclei during neutrophil migration and function in vivo. This project seeks to use the spatiotemporal resolution of a lattice light sheet microscope to examine this further, and explore its effect on neutrophil function. The project seeks to establish morphological and mechanical principles applying not just to neutrophils, but to all migratory cell types.