CULTURED CORNEAL EPITHELIUM--DRAIZE TEST REPLACEMENT

目前进行的最具争议的毒理学测试之一是 兔眼刺激性试验。除了道德上的强烈抗议 与程序相反，德雷兹测试受到了批评，因为 实验室间和实验室内的高度可变性，其缺乏 客观性，与之相比，兔眼的额外敏感性 对人类的眼睛来说。尽管如此，德雷兹考试已经成为标准 眼部毒理学检测方法发展近50年来 几年前。 这项研究的目的是开发一种替代Draze的方法 试验是眼毒性的可靠预测指标。建议数 Alternative是一种基于细胞培养的角膜模拟物，其细胞和 类似组织的行为可以用一个定义良好、可重现的 暴露在潜在的毒物或刺激物中的一种方式。 这样的系统将消除Draize方法的主观性， 增加重复性，避免非人道的道德问题 对动物的待遇。

One of the most controversial toxicology tests currently performed is the Draize rabbit eye irritancy test. In addition to the moral outcry against the procedure, the Draize test has been criticized due to the high degree of inter- and intra-laboratory variability, its lack of objectivity, and the extra sensitivity of the rabbit eye in comparison to the human eye. Nonetheless, the Draize test has become the standard ocular toxicological testing method since it was developed nearly 50 years ago. The purpose of this research is to develop an alternative to the Draize test which is a reliable predictor of ocular toxicity. The proposed alternative is a cell culture based corneal analog whose cellular and tissue-like behavior can be quantified in a well defined, reproducible manner as a function of exposure to a potential toxicant or irritant. Such a system would remove the subjectivity of the Draize method, increase reproducibility, and avoid the moral problems of inhumane treatment of animals.