HORMONES AND AGING--PITUITARY, AND HYPOTHALAMIC FUNCTION IN EXPERIMENTAL ANIMALS

猪瘟病毒分离的细胞和组织的分泌生理学 老年大鼠与成年雌雄大鼠垂体前叶的比较 已经在静态单层和动态灌流中进行了研究 体外培养系统。在单层培养中，暴露于 老年大鼠脑垂体细胞向离子载体A23187或佛波氏的迁移 Ester PMA，但不是三磷酸肌醇，基本上 减少了与年龄有关的赤字。在促性腺激素释放激素刺激的LM中 分泌物。接触A23187和PMA会导致协同作用 对成熟大鼠和老年大鼠细胞的影响。此外，还有 是与年龄相关的多巴胺对体外催乳素抑制的减少 从雄性和雌性大鼠的细胞中释放。然而，在那里 脑垂体膜多巴胺受体无增龄变化 性别间的结合，表明受体后的改变 是导致多巴胺作用随着年龄的增加而减少的原因 乳酸菌。在灌流培养中，结合下丘脑和 幼年成年大鼠的垂体前叶组织呈现节律性 PRL和ACTM的分泌，显然受因素(如 多巴胺和促肾上腺皮质激素释放激素)，提供了一种 未来老龄化研究的模型。分子遗传学实验 稳定的PRL mRNA没有随年龄增加而增加，而是随年龄增加而减少 在黄体生成素-β信使核糖核酸中， 去卵巢雌性大鼠。饮食和饮食的交互作用 雄性恒河猴睾丸的年龄和甲状腺功能的评估 还有松鼠猴子。

The secretory physiology of isolated cells and tissues from anterior pituitary glands of old vs. mature male and female rats has been studied in both static monolayer and dynamic perifusion in vitro culture systems. In monolayer culture, exposure of pituicytes from old rats to the ionophore A23187 or to the phorbol ester PMA, but not to inositol triphosphate, substantially diminished the age-related deficit. In GnRH-stimulated LM secretion. Exposure to both A23187 and PMA led to a synergistic effect in cells from both mature and old rats. In addition, there was an age-related decrease in dopamine inhibition of in vitro PRL release from cells of both male and female rats. However, there was no change with age in pituitary membrane dopamine receptor binding in either sex, suggesting that post-receptor alterations were responsible for the decreased dopamine action with age on the lactotrope. In perifusion culture, coupled hypothalami and anterior pituitary tissues from young adult rats exhibited rhythmic PRL and ACTM secretion, apparently modulated by factors (e.g. dopamine and CRH) from the hypothalamic fragments, providing a model for future aging studies. Molecular genetic experiments revealed no increase with age in stable PRL mRNA, but a decrease in LH-beta mRNA, in pituitary tissues and cells from intact and ovariectomized female rats. The interactive effects of diet and age on testis and thyroid function were evaluated in male rhesus and Squirrel monkeys.