SCREENING BRAIN DNA FOR HTLV-1 PROVIRAL SEQUENCES USING PCR AMPLIFICATION

聚合酶链式反应(PCR)扩增用于鉴定 多发性硬化症(MS)患者和患者的逆转录病毒样序列 非神经控制组患者。用聚合酶链式反应鉴定了一部小说 人脑DNA上的内源性逆转录病毒样序列，我们现在 确定其组织分布和转录活性。基因组学 从12例多发性多发性硬化症患者的尸检脑中提取DNA 并从8例硬化症患者的DNA中扩增出 执行，并使用克隆序列的放射性标记片段来 探测与人体不同器官的RNA互补的DNA，以及 来自不同的动物。聚合酶链式反应扩增与多发性硬化无关 被发现了。从聚合酶链式反应扩增产物中克隆DNA插入片段 与之前描述的任何序列不同，很可能 代表一个内源DNA序列。

Polymerase chain reaction (PCR) amplification has been used to identify retrovirus-like sequences in both multiple sclerosis (MS) patients and non-neurologic-control patients. Having used PCR to identify a novel endogenous retrovirus-like sequence on human brain DNA, we are now defining its tissue distribution and transcriptional activity. Genomic DNA was extracted from 12 autopsied brains of patients with multiple sclerosis and from eight without MS. PCR amplification of the DNA was performed, and radiolabeled fragments of cloned sequences were used to probe DNA complementary to RNA from different human organs, as well as from various animals. No correlation between PCR amplification and MS was found. The DNA insert cloned from PCR amplification products differed from any previously described sequence, and most likely represents an endogenous DNA sequence.