PHYSIOLOGICAL AND CYTOCHEMICAL PATHOLOGY OF PARASITIC DISEASES

研究了一种溶菌的纯化和化学特性， 抗锥虫因子（ATF-II）来自细菌种，假单胞菌 荧光，继续。 元素微量分析，反相高 高效液相色谱法，葡聚糖凝胶过滤，凝胶 电泳、快原子轰击质谱（FAB），以及 使用核磁共振光谱（NMR）。 纯化的 ATF-II由三种主要成分组成，α-d-糖苷，肽， 还有一种成分没有被识别出来，但似乎是一种污染物。 正在使用更精确的HPLC来消除该馏分并促进 化学结构的精确测定。 测定 在合成研究之前，化学结构是必不可少的。 引发和药理学和毒理学性质进行了检查 利用克氏锥虫感染。

Studies on the purification and chemical characterization of a lytic, anti-trypanosomal factor (ATF-II) from the bacterial species, Pseudomonas fluorescens, were continued. Elemental microanalysis, reversed-phase high performance liquid chromatography (HPLC), Sephadex gel filtration, gel electrophoresis, fast atom bombardment mass spectroscopy (FAB), and nuclear magnetic resonance spectroscopy (NMR) were employed. The purified ATF-II consists of three main components, an alpha-d-glycoside, a peptide, and a component that was not identified, but appears to be a contaminant. More precise HPLC is being used to eliminate this fraction and facilitate an exact determination of the chemical structure. The determination of the chemical structure is essential before studies on synthesis are initiated and pharmacological and toxicological properties are examined employing Trypanosoma cruzi infections.