CHARACTERIZATION OF A NOVEL HTLV II CELL LINE

我们以前分离的细胞系（JR）从患者再生障碍性贫血， 产生HTLV II的贫血。 我们将继续分析 细胞系和它产生的病毒，以确定是否 它与发育不全的联系是由于这种独特的特征， 孤立。 我们用Western blot分析JR细胞产生的蛋白质 分析JR细胞和纯化的病毒，并将这些结果与 标准HTLV II细胞和病毒。 JR细胞DNA扩增片段的PCR序列分析 准备并测序。 这些结果显示了广泛的同源性 JR和已知的HTLV II分离株之间的差异。 我们正在克隆JR病毒。 来自JR细胞基因组DNA的EcoRI片段 并克隆到λ载体中。 筛选文库 使用HTLV II Mo病毒DNA的亚克隆。 阳性克隆 已鉴定并部分测序。 我们的研究结果表明， JR和已知的HTLV II分离株之间的广泛同源性。 我们 继续克隆病毒 我们分析了其他再生障碍性贫血患者的血清中的抗体 HTLV I/II。 检测到一个额外的反应性样品。 我们 目前正在研究这个人是否感染了HTLV。

We previously isolated a cell line (JR) from a patient with aplastic anemia that produces HTLV II. We are continuing to characterize this cell line and the virus that it produces in order to determine whether its association with aplasia is due to a unique characteristic of this isolate. We analyzed the proteins produced by JR cells by Western blot analysis of JR cells and purified virus and compared these results with standard HTLV II cells and viruses. PCR based sequence analysis of DNA fragments amplified from JR cells were prepared and sequenced. These results show extensive homology between JR and known HTLV II isolates. We are cloning JR virus. Eco RI fragments from genomic DNA of JR cells were prepared and cloned in a lambda vector. The library was screened using subclones of HTLV II Mo viral DNA. Positive clones were identified and partially sequenced. Our results indicate that there is extensive homology between JR and known HTLV II isolates. We are continuing our efforts to clone this virus. We have analyzed sera from other aplastic anemia patients for antibodies to HTLV I/II. One additional reactive sample was detected. We are currently studying whether this person was infected with HTLV.