COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

分析方法正在继续开发和应用，使用的是 GELLAB-II软件系统--探索性数据分析系统 对多组二维电泳胶图像进行分析。它包含了 用于图像采集、处理、数据库的复杂子系统 操纵、图表和统计分析。它已被应用于 各种实验系统，其中定量和定性 在成百上千种未改变的蛋白质中，一种或多种蛋白质的变化 蛋白质是基本的分析问题。跟踪更改 使用这些方法进行检测也是该系统的一个主要属性。一个 复合凝胶数据库可以在不同的勘探数据下进行查看 分析条件和统计差异以及微妙的模式 从一个有效的3D数据库的“切片”中阐明。结果可以是 以各种表格、曲线图或派生图像等形式呈现 广域网上的工作站。 实质性应用包括：正在进行的镉毒性研究 尿液(Myrick)、雷特综合征(Myrick)、佛蒙特州汞研究(Myrick)、 血清二恶英研究(Myrick)：用于鉴别的2D DNA凝胶 表达的基因(Rogan等人)；切尔诺贝利的放射性沉降物 (Krekling)；艾滋病疫苗计划/FCRDC使用图像处理来改进： HIV-1蛋白凝胶定量分析及Western印迹分析 (McGrath等人)。GELLAB-II已出口到：CDC/亚特兰大(Myrick)， 大学。挪威(Krekling)，大学苏黎世(桑德雷格)。今年，疾控中心已经 是GELLAB-II的主要Beta测试点。对以下内容的其他更改 帮助继续与增强版协作并将其导出 GELLAB的图形界面版本一直被积极追求，并且 今年的主要活动。

Analytic methods are continuing to be developed and applied using the GELLAB-II software system - an exploratory data analysis system for the analysis of sets of 2D electrophoretic gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images and on workstations over wide area networks. Substantive applications include: Ongoing studies of cadmium toxicity in urine (Myrick), Rett syndrome (Myrick), Vermont mercury study (Myrick), serum dioxin study (Myrick); 2D DNA gels for identifying differentially expressed genes (Rogan et al.); radioactive fall-out from Chernobyl (Krekling); Aids Vaccine Program/FCRDC using image processing to improve: quantitative analysis of HIV-1 protein gels and Western blot assays (McGrath et al.) . GELLAB-II had been exported to: CDC/Atlanta (Myrick), Univ. Norway (Krekling), Univ. Zurich (Sonderegger). This year, CDC has been the primary Beta test site for GELLAB-II. Additional changes to help continued collaboration with and exporting of enhanced graphical-interface versions of GELLAB has been actively pursued and the primary activity this year.