PHYSIOLOGICAL AND CYTOCHEMICAL PATHOLOGY OF PARASITIC DISEASES

一种抗锥虫因子(ATF-1)的纯化研究 Ii)从细菌种荧光假单胞菌中分离到 继续。有必要修改提纯程序。 以前用于获得纯分数，这些分数是 适用于核磁共振分析。和以前一样，我们使用了高速 采用ITO多层膜的逆流色谱(CCC) 盘状色谱柱，但在高压层析系统(HPLC)中 我们采用了制备性和分析性碳水化合物分析。 柱，以获得核磁共振组分。工作正在进行中，以获得 足够数量的纯化合物用于这些研究。这个 化学结构的确定是研究前必不可少的。 合成是启动的，药理学和毒理学 使用实验性感染来检查属性 克氏锥虫。

Studies on the purification of an anti-trypanosomal factor (ATF- II) from the bacterial species, Pseudomonas fluorescens, were continued. It was necessary to modify the purification procedure used previously in order to obtain pure fractions that were suitable for NMR analysis. As before, we used high-speed countercurrent chromatography (CCC) employing the Ito multilayer coiled column but in the high pressure chromatography system (HPLC) we employed both preparative and analytical carbohydrate analysis columns to obtain the NMR fractions. Work is in progress to obtain sufficient amounts of pure compound for these studies. The determination of chemical structure is essential before studies on synthesis are initiated and pharmacological and toxicological properties are examined employing experimental infections with Trypanosoma cruzi.