COLLAGEN CRIMPING AND FIXATION PRESSURE IN PORCINE AORTIC VALVES

生物组织中的胶原卷曲或波浪状可以是 以幅度和周期的方式进行定量测量 偏振光显微镜和电子显微镜。在猪主动脉中 为用作生物人工心脏瓣膜而准备的瓣膜，这种卷曲 显示了受Low组织固定条件的影响 戊二醛浓度：无压力固定时 应用时，其卷曲程度与自体主动脉瓣相似； 然而，低压力固定导致显著减少卷曲 大部分的胶原蛋白和高压固定几乎消除了它 完全在脊髓和中尖区。一种技术已经被 为在完整的主动脉叶上进行这些观察而开发的。

The crimping or waviness of collagen in biological tissues can be measured quantitatively in terms of amplitude and periodicity by means of polarized light microscopy and electron microscopy. In porcine aortic valves prepared for use as bioprosthetic cardiac valves, this crimping was shown to be influenced by the conditions of tissue fixation with low concentrations of glutaraldehyde: when fixation was used with no pressure applied, the crimping was similar to that in the native aortic valve; however, low pressure fixation caused marked reduction in the crimping of much of the collagen, and high pressure fixation almost eliminated it completely in the cords and the mid-cusp region. A technique has been developed for making these observations on intact aortic leaflets.