SOURCES AND EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN THE BRAIN

在小胶质细胞上进行了实验，小胶质细胞是驻留在 中枢神经系统，从大鼠大脑皮层培养而来。我们 此前已发现钙离子可以进入小胶质细胞 当这些细胞超极化时。我们现在已经发现，使用膜片钳 电极上存在L型电压依赖性钙通道 当细胞去极化时，细胞变得开放。我们还发现，一个 浓度为25um的亚铁离子作用3小时后对小胶质细胞有毒性作用。 因为我们之前已经证明小胶质细胞可以产生超氧化物 自由基离子，我们认为亚铁离子的毒性机制是 提高铁催化产生羟基自由基的能力 超氧阴离子。

Experiments have been performed on microglia, the resident macrophage in the central nervous system, cultured from cerebral cortices of rat. We have previously found that the calcium ion can enter the microglial cells when these cells are hyperpolarized. We have now found, using patch-clamp electrodes, that there are L-type voltage-dependent calcium channels that become open when cells are depolarized. We have also found that a concentration of 25 uM ferrous ion is toxic to microglia after 3 hours. Since we have previously shown that microglia can produce the superoxide radical ion, we believe that the mechanism of ferrous ion toxicity is due to the enhanced iron catalytic production of the hydroxyl radical from the superoxide radical ion.