ROLE OF NEUROPEPTIDES AND BIOGENIC AMINES IN NEUROENDOCRINE REGULATION

拟精神病药物二甲氧基苯基异丙胺的受体 (DOI)在离散的大脑区域被发现，并被证明是交叉的 用迷幻药取代。这些受体被证明是5HT2 打字。大鼠长期服用DOI后，其体重下降 皮质5HT2受体的调节。新的非常敏感 建立了定量放射免疫细胞化学方法。这些 方法允许定位和量化许多 组织切片中单个大鼠脑核中的抗原，通过 放射自显影和定量显微密度测定仪，并有100- 比经典放射免疫分析的灵敏度高一倍。 定量放射自显影用于胺类化合物的研究 以及人类淋巴细胞和血小板中的神经肽受体， 灵敏度比以前的检测方法提高了十倍。 我们的室旁核和促肾上腺素发生了深刻的变化 大鼠CRF分泌改变条件下的II受体， 提示中枢和外周血管紧张素在慢性肾功能衰竭中的作用 释放压力，释放压力。我们已经描述和描述了 血管紧张素II和心钠素受体 大鼠的交感神经节，发现这些神经节有变化 受体在交感神经活动改变的情况下，提示 这些外周/局部激素在调节 自主神经功能。 我们应用定量放射自显影技术来研究 不连续脑区的β-肾上腺素能受体的分类 心脏传导系统的一部分。

Receptors for the psychotomimetic dimethoxyphenylisopropylamine (DOI) were identified in discrete brain areas and shown to cross- displace with LSD. These receptors were shown to be of the 5HT2 type. Chronic DOI administration to rats resulted in a down regulation of cortical 5HT2 receptors. New very sensitive quantitative radioimmunocytochemical methods were developed. These methods allowed for the localization and quantification of many antigens in individual rat brain nuclei in tissue sections, by autoradiography and quantitative microndensitometry and have a 100- fold higher sensitivity than classical RIA. Quantitative autoradiography was utilized for the study of amine and neuropeptide receptors in human lymphocytes and platelets, with a ten-fold increase in sensitivity over previous assays. We have profound changes in paraventricular nucleus angointensin II receptors in conditions of altered CRF secretion in rats, suggesting a role for central and peripheral angiotensin in CRF release and in stress. We have described and characterized angiotensin II and atrial natriuretic peptide receptors in sympathetic ganglia of the rat and found alterations in these receptors in conditions of altered sympathetic activity, suggesting a role for these peripheral/local hormones in the regulation of autonomic function. We applied quantitative autoradiographic techniques to study the classification of beta-adrenocreptors in discrete brain areas and parts of the conduction system of the heart.