LIPID METABOLISM OF THE MACACA FASCICULARIS

这项研究将通过以下方式检查外源脂肪酸的代谢 短束猕猴。不能合成的反式脂肪酸 由真核生物将其作为外源脂肪酸的标记。 这些脂肪酸是顺式脂肪酸的几何异构体， 可以由真核生物合成。反式脂肪酸是 在脂肪酸之后不久就分解代谢并从体内移走 从饮食中移除。12只雄性束支原体猴子 在研究中使用了。六个人被喂以对照饮食，而 其他人则接受实验饮食。猴的红细胞膜 饲喂含有0.5%反式十八酸异构体的对照日粮 脂肪酸。它们粪便中脂肪酸的检测 猴子表明，生物氢化确实发生在它们的肠道中 产生反式脂肪酸，它似乎被合并到 红细胞膜。在启动研究后的6周内 饲喂实验饲料的猕猴红细胞膜含量为10.8% 总脂肪酸的反式十八酸异构体。 与反式脂肪酸的增加相对应的是 膜上亚油酸与花生四烯酸的比例增加。这 表明反式脂肪酸干扰花生四烯酸 综合。饱和脂肪酸也被取代了。 顺式十八酸异构体。双债券指数为 计算并表明，观测到的微扰在 猕猴红细胞膜脂肪酸含量的研究 实验日粮导致膜流动性降低。

The study will examine the metabolism of exogenous fatty acids by Macaca fascicularis. Trans fatty acids which cannot be synthesized by eukaryotes will be used as the exogenous fatty acid marker. These fatty acids are geometrical isomers of cis fatty acids which can be synthesized by eukaryotes. The trans fatty acids are catabolized and removed from the body shortly after the fatty acids are removed from the diet. Twelve male M. fascicularis monkeys were used in the study. Six were fed the control diet, and the others received the experimental diet. The RBC membrane of monkeys fed the control diet contained .5% trans octadecanoate isomers of the fatty acids. Examination of the fecal fatty acids from these monkeys showed that biohydrogenation does occur in their gut producing trans fatty acids which appear to be incorporated into the RBC membrane. Within 6 weeks after initiating the study the RBC membranes of monkeys fed the experimental diet contained 10.8% trans octadecanoate isomers of the total fatty acids. Corresponding with the increase in trans fatty acids there was an increase in the membrane linoleate to arachidonate ratio. This indicated that trans fatty acids interfere with arachidonate synthesis. There was also a displacement of saturated fatty acids and cis octadecanoate isomers. The double bond index was calculated and indicated that the observed perturbations in the fatty acid content of the RBC membranes from monkeys fed the experimental diet resulted in decreased membrane fluidity.