INVESTIGATIONS OF ATPASE AND OUABAIN-LIKE FACTOR IN EPILEPSY

据报道，Na+-K+ATPase和哇巴因结合是 在点燃的癫痫大鼠模型中降低。Na+-K+ATPase 参与将膜电位维持在65 mV。一个 酶活性的降低会导致去极化。 在细胞膜上。我们的工作将涉及调查 Na~+-K~+-ATPase系统在灌浆和非灌浆中的地位 通过测量人的颞叶组织的尖峰 酶的活性以及哇巴因的结合。一个 在这些研究中，将尝试证明一种改变 在酶的活性上。 点燃大鼠脑内Na~+-K~+-ATPase活性不变 哇巴因在人类大脑皮层中的结合没有改变。这样的一个 缺乏改变可能表明ATPase异常发生在 极少量的电池，不能用电流检测到 程序。 以确定ATPase是否在癫痫组织中起作用 动物和人脑、点燃的大鼠和人脑将 评估ATPase活性以及哇巴因结合活性。 高血压病患者体内Na~+-K~+-ATPase活性的研究 点燃大鼠大脑和人类癫痫灶已经完成。 在人类中发现ATPase活性没有差异 焦点与非焦点。它们之间没有区别 组织并不意味着缺乏这种酶的参与 癫痫灶。找不到的原因之一 不同之处可能是酶活性的变化 可能发生在大脑尖峰区域的微小区域 并且不能用目前可用的方法来检测。

Na +-K + Atpase and ouabain binding have been reported to be decreased in the kindled rat model of epilepsy. Na + -K + ATPase is involved in maintaining the membrane potential at 65mV. A decrease in the activity of the enzyme will cause depolarization of the cell membrane. Our work will involve the investigation of the status of the Na + -K + ATPase system in spiking and non spiking tissue from the human temporal lobe by measuring the activity of the enzyme as well as the binding of ouabain. An attempt will be made in these studies to demonstrate an alteration in the activity of the enzyme. Na + -K + ATPase activity is not altered in the kindled rat brain and ouabain binding is not altered in the human cortex. Such a lack of alteration may indicate that ATPase abnormalities occur in a very small number of cells and cannot be detected with current procedures. To determine if the ATPase has a role in the epileptic tissue from animals and human brain, kindled rats and human brain will be evaluated for ATPase activity as well as ouabain binding activity. The investigation of the activity of Na + -K + ATPase in the kindled rat brain and the human epileptic focus has been completed. No difference in the activity of ATPase were found in the human focus versus the nonfocus. The lack of difference between these tissues does not indicate a lack of involvement of this enzyme in the epileptic focus. One reason for the inability to find a difference could be that the alteration of the enzyme activity could be occurring in a minuscle area of the spiking brain region and cannot be detected with the presently available methods.