CELLULAR BIOCHEMISTRY STUDIES ON CHEMICAL SELECTED FOR EVALUATION BY NTP

无机或有机金属及金属络合物的作用 NTP特别感兴趣，因为他们的 饮用水和工业过程中的流行度，用作 抗癌药物中的成分及其不同的靶点 器官中毒。 细胞免疫毒性的机制在大鼠或 小鼠暴露于氯化汞、硫酸镍或 二氯二茂钛。这些金属在体内的组织积累 靶器官显示了生化的敏感性 评估靶器官毒性的试验通常先于 大体、显微或临床方法。动物的发育 研究Fischer大鼠白血病的模型揭示了肿瘤标志物 对于这种疾病可以用来区分 AGE诱导和化学增强的白血病发生。尿液 评估对肾毒性化学物质损伤的酶反应。 慢性肾功能衰竭患者肾毒性的预测模型 学习。酶促方法的发展以提高稳定性， 敏感度和效率 7-乙氧基香豆素-邻位脱甲基酶用于评价 微量化学物质的MFO-引发活性 啮齿动物组织。

The effect of inorganic or organic metals and metal complexes is of particular interest to the NTP because of their prevalence in drinking water and industrial processes, use as constituents in anticancer drugs, and their diverse target organ toxicities. Mechanisms of cellular immunotoxicity were studied in rats or mice exposed to mercuric chloride, nickel sulfate or titanocene dichloride. Tissue accumulation of these metals in target organs indicated the sensitivity of the biochemical assays to evaluate target organ toxicity which often preceeded gross, microscopic or clinical methods. Development of animal models to study Fischer rat leukemia revealed tumor markers for this disease that can be use to distinguish between age-induced and chemically-enhanced leukemogenesis. Urinary enzyme responses to nephrotoxic chemical insult were evaluated to use as a model to predict renal toxicity in chronic studies. Enzymatic method development to enhance stability, sensitivity, and efficiency of 7-ethoxycoumarin-ortho-demethylase was initiated to evaluate MFO-initiating activity of chemicals in minute amounts of rodent tissue.