ROLE OF NEUROPEPTIDES AND BIOGENIC AMINES IN NEUROENDOCRINE REGULATION

我们研究了几种神经肽(血管紧张素II、心房肌 利钠因子，P物质)和生物胺(多巴胺， 去甲肾上腺素、5-羟色胺)在中枢调节中的作用 自主神经系统和脑下垂体。特别强调的是 用于心血管中枢控制和体液中枢控制 新陈代谢。 生物胺在单个大脑核团中的代谢 放射酶分析和高压液相色谱检测。 用放射免疫法测定脑核神经肽含量。 神经肽和生物胺受体在个体大脑中的研究 原子核计算机定量放射自显影方法 显微密度测定法和与125I标准的比较。包括动物模型 自发性(遗传)高血压大鼠(SHR)，Brattleboro大鼠， 不能合成加压素，神经源性高血压大鼠 (去窦弓神经)，急性脱水大鼠和肾上腺切除， 去肾上腺髓质和去垂体大鼠。 我们证明了血管紧张素II和心钠素的中心作用。 遗传性高血压和脱水的因素以及生物性高血压的作用 胺、多巴胺、去甲肾上腺素和5-羟色胺与遗传性高血压和 在昼夜节律的调节中。

We studied the role of several neuropeptides (angiotensin II, atrial natriuretic factor, substance P) and biogenic amines (dopamine, norepinephrine, serotonin) in the central regulation of the functions of the autonomic nervous system and the pituitary gland. Special emphasis was given to central cardiovascular control and the central control of fluid metabolism. The metabolism of biogenic amines in individual brain nuclei was studied by radioenzymatic assays and by high pressure liquid chromatography. Neuropeptide content of brain nuclei was studied by radioimmunoassays. Neuropeptide and biogenic amine receptors were studied in individual brain nuclei by quantitative autoradiographic methods with computerized microdensitometry and comparison to 125I-standards. Animal models included the spontaneously (genetic) hypertensive rat (SHR), the Brattleboro rat, unable to synthesize vasopressin, neurogenically hypertensive rats (sinoaortic denervated), acutely dehydrated rats and adrenolectomized, adrenodemedulectomized and hypophysectomized rats. We demonstrated a central role for angiotensin II and atrial natriuretic factor in genetic hypertension and dehydration, and a role for the biogenic amines, dopamine, norepinephrine, and serotonin in genetic hypertension and in the regulation of circadian rhythms.