CORE--TISSUE PROCUREMENT

组织核心设施将作为所有患者的储存库 包括外周血淋巴细胞所需的样本 口腔癌研究中心的不同研究项目。这个 设施将负责采购、储存和分发 组织学特征的样本来自不同的病变，包括 口腔鳞状细胞瘤的组织病理学特征。详细测绘 对每个样本进行仔细的采样和验证过程 以确定每个损伤的性质和范围将在 每个样本。标准化的编码、处理、保存和库存 将对标本进行检查，以确保统一和标准化 处理，以确保患者的机密性并避免分析偏差。 该核心还将负责集中处理和 提取蛋白质、RNA和DNA以最大限度地提高组织利用率 小而有限的标本。流式细胞仪分析变异体 包括增殖、Bcl-2和细胞凋亡等参数以及 癌基因、肿瘤抑制基因和 还将根据具体项目的要求进行细胞标记。

The tissue core facility will function as a repository for all patients specimens including peripheral blood lymphocytes that are required for the different research projects of the oral cancer research center. The facility will be responsible for acquisition, storage and distribution of histologically characterized samples from different lesions comprising the histopathological spectrum of oral squamous neoplasia. Detailed mapping of each specimen followed by a careful sampling and verification process to determine the nature and the extent of each lesion will be conducted on each sample. Standardized coding, processing, preservation and inventory of the specimens will be carried out to ensure uniform and standardized handling, to secure patient confidentiality and avert analytical bias. The core will also be responsible for centralized processing and extraction of protein, RNA, and DNA to maximize tissue utilization from small and limited specimens. Flow cytometric analysis of varying parameters including proliferation, Bcl-2 and apoptosis, as well as immunohistochemical staining for oncogenes, tumor suppressor genes, and cellular markers will also be performed as requested by specific projects.