CORE--TISSUE PROCUREMENT

组织核心设施将作为所有患者的储存库 包括外周血淋巴细胞在内的标本， 口腔癌研究中心的不同研究项目。 的 工厂将负责采购、储存和分发 来自不同病变的组织学表征的样品，包括 口腔鳞状细胞瘤组织病理学谱。 详细映射 每一个样本，然后仔细取样和验证过程 以确定每个病变的性质和程度， 输液袋的 标准化编码、处理、保存和库存 将进行标本的统一和标准化 处理，以确保患者的保密性和避免分析偏倚。 核心还将负责集中处理， 提取蛋白质，RNA和DNA，以最大限度地利用组织， 小而有限的标本。 流式细胞术分析不同的 包括增殖、Bcl-2和凋亡的参数，以及 癌基因、肿瘤抑制基因的免疫组织化学染色， 还将根据具体项目的要求进行细胞标记。

The tissue core facility will function as a repository for all patients specimens including peripheral blood lymphocytes that are required for the different research projects of the oral cancer research center. The facility will be responsible for acquisition, storage and distribution of histologically characterized samples from different lesions comprising the histopathological spectrum of oral squamous neoplasia. Detailed mapping of each specimen followed by a careful sampling and verification process to determine the nature and the extent of each lesion will be conducted on each sample. Standardized coding, processing, preservation and inventory of the specimens will be carried out to ensure uniform and standardized handling, to secure patient confidentiality and avert analytical bias. The core will also be responsible for centralized processing and extraction of protein, RNA, and DNA to maximize tissue utilization from small and limited specimens. Flow cytometric analysis of varying parameters including proliferation, Bcl-2 and apoptosis, as well as immunohistochemical staining for oncogenes, tumor suppressor genes, and cellular markers will also be performed as requested by specific projects.