3 DIMENSIONAL STRUCTURE OF IRE IN FERRITIN MRNA, STUDIED BY NMR SPECTROSCOPY

SIR提供了L-氨基胍-13C，“N_2；15 mg ‘c，’N RNA；1.2克 13C，“N RNA；292 mg 13C，“N RNA；320 mg 560毫克真核基因中的IRE顺式元件高度 保守，并具有特征性的特定调节蛋白。我们的 功能和结构的研究，使用定点突变和 以择形过渡金属络合物为结构的解理 探头，表明折叠结构可能具有不寻常的三级结构 功能。我们的初步核磁共振波谱显示，IRE 序列(30聚体)在溶液中形成单一构象， 便于结构分析。富13C和15N的使用 无论是否有统一标记的核糖核苷酸，对 核磁共振研究。

The SIR provided L-AMinine-guanido- 13C, "N2; 15mg 'C,'N RNA; 1.2g 13C, "N RNA; 292mg 13C, "N RNA; 320mg "C, "N RNA; 560mg The IRE cis element in eukaryotic mRNA is highly conserved and has a characterized specific regulator protein. Our studies of function and structure, using site-directed mutagenesis and cleavage by shape-selective transition metal complexes as structure probes, indicate a folded structure with possibly unusual tertiary features. Our preliminary NMR spectroscopy shows that the IRE sequence (a 30 mer) forms a single conformer in solution which facilitates the structure analysis. The use of 13C- and 15N-enriched ribonucleotides, uniformly labelled or otherwise, are crucial to the NMR study.