GENOMIC ALTERATIONS IN PROSTATE CANCER

波瓦博士和他在约翰霍普金斯大学的同事们做了大量的工作 前列腺癌的基因组改变的研究，并发现了一个 某些染色体缺失或增加的强烈趋势;其他 染色体很少缺失或获得。 他们还研究了 前列腺癌的端粒长度 他们的假设是， 特定染色体上的丢失事件的倾向可能与 染色体特异性端粒长度差异。 最好是 在直接从病人身上分离的标本中研究这些长度， 然而，由于中期染色体难以获得， 从这些样本中，我们使用前列腺癌细胞系。 染色体#13和#19（对照）已被分类并发送给Dr. Griffith使用他非常敏感的Southern分析进行端粒分析 这需要少得多的已分类染色体。 显著差异 已被报道。 不同时期的正常人包皮细胞 他们的生活史也被以类似的方式分析， 建立染色体与染色体端粒差异作为函数 细胞培养进展。 替代检测方法也正在 目前评价。

Dr. Bova and colleagues at Johns Hopkins have done extensive studies of genomic alterations in prostate cancer and have found a strong tendency for certain chromosomes to be deleted or gained; other chromosome are rarely deleted or gained. They have also studied telomere length in prostate cancer. Their hypothesis is that the tendency toward loss events on specific chromosomes may be related to chromosome-specific telomere length differences. It would be best to study these lengths in specimens isolated directly from patients, however, because metaphase chromosome are difficult to obtain in quantity from these samples, we are using prostate cancer cell lines. Chromosomes #13 and #19 (control) have been sorted and sent to Dr. Griffith for telomere analysis using his very sensitive Southern assay which requires far fewer sorted chromosomes. Significant differences have been reported. Normal human foreskin cells at different stages their life history are als o being analyzed in a similiar manner to establish chromosome to chromosome telomere differences as a function of cell culture progression. Alternative assays are also being evaluated at the present time.