A REPLACEMENT CELL SORTER FOR PURDUE UNIVERSITY

本申请旨在购买一台新的细胞分选机，以取代现有的旧分选机，旧分选机不再可靠，无法升级到当前标准，也无法满足当前用户的许多要求。在过去的14年里，普渡大学一直保持着细胞分选机的运作。1986年，Epics 752在NIH共享仪器补助金下购买。该仪器于1994年完全停止使用。1990年，根据与制造商的协议，我们获得了Epics Elite分拣机的原型，该分拣机在过去9年中一直为该工厂服务。由于该仪器不是完整的生产型号，因此该仪器的某些方面不符合标准。此外，该仪器即将达到其使用寿命的终点，并且随着我们机构对流式细胞术分选方面的需求增加，我们认为现在是用能够满足用户需求的仪器取代该仪器的时候了。在这方面，所提出的仪器已被设计为不仅执行高速分选，而且快速设置分选会话，从12-384孔板范围内的优秀单细胞板沉积单元，以及我们目前需要或未来几年内当前和未来用户可能需要的所有激光组合。该设施在共享使用我们的高科技仪器方面有着悠久的历史。他们经营的资源有着丰富的历史，不仅为该机构的NIH研究人员提供资源，而且为全国各地机构的研究人员提供知识和教育资源。所要求的仪器将为我们的资源提供急需的升级，为NIH资助的研究人员提供产生高质量科学所需的高质量工具。

This application is to purchase a new cell sorter to replace an existing, older sorter which is no longer reliable, cannot be upgraded to current standards, and cannot perform a number of requirements of current users. Over the past 14 years Purdue University has maintained a cell sorter operation. In 1986 an Epics 752 was purchased under an NIH shared instrumentation grant. This instrument was taken completely out of service in 1994. In 1990, under an agreement with the manufacturer, we obtained a prototype Epics Elite sorter which has served this facility well over the past 9 years. Because this instrument was not a full production model, there are certain aspects to the instrument that are not standard. In addition, the instrument is close to reaching the end of its useful life and, with added demands upon the sorting aspects of flow cytometry at our institution, we believe it is time to replace this instrument with an instrument that can accommodate our user's needs. In this regard the instrument proposed has been designed to perform not only high speed sorts, but rapid set-up sort sessions, an excellent single cell plate deposition unit ranging from 12-384 well plates, and all of the laser combinations we currently require or &e likely to need with current and future users over the next several years. The facility has a long track record of shared use of our high technology instruments. The resource in which they are operated has a rich history of not only providing resources to NIH investigators at this institution, but also knowledge and educational resources to those at institutions all over the country. The instrument requested will provide a much needed upgrade to our resource providing NIH funded investigators with the high quality tools necessary to produce quality science.