Cell based biosensor fabricated by soft Lithography

软光刻技术是在微米尺度上操纵和图案生物材料的有力工具。这些方法允许在定义明确的、受限的表面区域进行化学和生物学研究。微接触印刷和微流体通道的图案将结合起来制造基于细胞的生物传感器，这些传感器将进一步用于研究细胞行为。这种生物测定最终将有助于疾病的检测和分析。细胞生物测定的能力和有效性将通过细胞蛋白粘附和吸附的概念验证演示进行测试。此外，这种基于细胞的生物传感器将能够研究牛毛细血管内皮细胞（BCE）的血管生成和血管形成。由于血管生成生长因子和抑制剂的存在，细胞形态的变化将在单个芯片上的平行通道中进行研究。此外，分化，毛细血管的形成，在BCE细胞将被研究在图案粘合剂底物。最后，我们将探讨BCE细胞和肿瘤细胞之间的相互作用，以了解它们的扩散如何受到血管生成启动子或抑制子的影响。这些最初的实验应该提供对所提出的生物测定的潜力和局限性的见解，以及如何利用这种装置来研究未知生物活性分子对细胞的影响。

Soft lithographic techniques are powerful tools to manipulate and pattern biomaterials on the micrometer scale. These methods allow chemical and biological studies on well-defined, confined regions of surfaces. Microcontact printing and patterning by microfluidic channels will be combined to fabricate cell-based biosensors that will be further utilized to investigate cell behavior. This bioassay will ultimately assist in detection and analysis of diseases. The capabilities and effectiveness of the cell bioassay will be tested through proof of concept demonstrations of cell-protein adhesion and adsorption. Also, this cell-based biosensor will enable investigations of angiogenesis and blood vessel formation in bovine capillary endothelial (BCE) cells. Changes in cell morphology due to the presence of angiogenic growth factors and inhibitors will be studied in parallel channels on a single chip. Moreover, differentiation, capillary tube formation, in BCE cells will be investigated on patterned adhesive substrates. Finally, interactions between BCE cells and tumor cells will be probed to learn how their spreading is affected by the presence of either angiogenic promoters or suppressors. These initial experiments should provide insight on the proposed bioassay's potential and limitations, and how such a device may be exploited to investigate the effects of unknown biologically active molecules on cells.