GENOMIC ALTERATIONS IN PROSTATE CANCER

博瓦博士和他在约翰·霍普金斯大学的同事们已经做了大量的 对前列腺癌基因组改变的研究发现 某些染色体有强烈的缺失或获得倾向；其他 染色体很少被删除或获得。他们还研究了 前列腺癌的端粒长度。他们的假设是 在特定染色体上发生丢失事件的倾向可能与 染色体特有的端粒长度差异。最好的办法是 在直接从患者身上分离的样本中研究这些长度， 然而，由于中期染色体在中国很难获得 从这些样本中，我们使用的是前列腺癌细胞系。 染色体#13和#19(对照)已经被分类并送到Dr。 格里菲斯用他非常灵敏的Southern分析端粒 这需要的排序染色体要少得多。显著差异 已经被报道过。不同发育阶段的正常人包皮细胞 他们的生活史也是以类似于 将染色体与染色体的端粒差异作为一种函数 对细胞培养进程的影响。替代化验也在进行中 目前进行了评估。

Dr. Bova and colleagues at Johns Hopkins have done extensive studies of genomic alterations in prostate cancer and have found a strong tendency for certain chromosomes to be deleted or gained; other chromosome are rarely deleted or gained. They have also studied telomere length in prostate cancer. Their hypothesis is that the tendency toward loss events on specific chromosomes may be related to chromosome-specific telomere length differences. It would be best to study these lengths in specimens isolated directly from patients, however, because metaphase chromosome are difficult to obtain in quantity from these samples, we are using prostate cancer cell lines. Chromosomes #13 and #19 (control) have been sorted and sent to Dr. Griffith for telomere analysis using his very sensitive Southern assay which requires far fewer sorted chromosomes. Significant differences have been reported. Normal human foreskin cells at different stages their life history are als o being analyzed in a similiar manner to establish chromosome to chromosome telomere differences as a function of cell culture progression. Alternative assays are also being evaluated at the present time.