ASSEMBLY AND FUNCTION OF THE HERPES SIMPLEX ENVELOPE

单纯疱疹病毒（HSV）包膜的形成和排出 从细胞中分离包膜病毒体，对于产生感染性 粒子和疾病的传播。不幸的是，很少有人知道HSV是如何 衣壳和被膜蛋白质将细胞膜重塑为被膜。 类似地，用于病毒的细胞质细胞器的身份 胞吐作用仍不清楚。我们的工作模型是HSV衣壳获得它们的 最后通过附着在高尔基体表面而被包裹在细胞质中 或者说，他们是一群人，或者说，他们是一群人，或者说他们是一群人。使用新的分析技术 在这个实验室开发的，我们将纯化，然后建立身份， 用于HSV抑制的细胞质细胞器的功能。机制 衣壳和被膜蛋白与这些表面的关联 细胞器是病毒繁殖的第一步，然后将确定。 最后，衣壳与细胞质区室的相互作用将是 通过对绿色荧光物质的运输进行成像， 蛋白质标记的衣壳在一个单一的，同步的组装波。 从这项研究中获得的信息将有助于设计能够 干扰HSV的传播，从而降低这种严重的 人类病原体

Formation of the herpes simplex virus (HSV) envelope, and egress of enveloped virions from cells, is critical for the production of infectious particles and the spread of disease. Unfortunately, little is know of how HSV capsids and tegument proteins refashion cell membranes into envelopes. Similarly, the identity of the cytoplasmic organelles utilized for viral exocytosis remains obscure. Our working model is that HSV capsids acquire their final envelope in the cell cytoplasm by attaching to the surface of the Golgi apparatus or endosomes, then budding into them. Using novel assay techniques developed in this laboratory, we will purify then establish the identity and function of cytoplasmic organelles utilized for HSV envelopment. The mechanism of association of capsids and tegument proteins with the surface of these organelles, the first step in viral envelopment, will then be determined. Finally, the interaction of capsids with cytoplasmic compartments will be visualized in living cells by imaging the trafficking of Green Fluorescent Protein tagged capsids during a single, synchronized wave of assembly. Information obtained from this study will help in the design of agents able to interfere with HSV envelopment, thus reducing the virulence of this serious human pathogen.