Human Tissue Model For Heterosexual HIV Transmission

描述：(申请者提供)第一阶段研究适应原代培养 方法获得可靠的正常宫颈外阴道(ECV)来源 上皮细胞和朗格汉斯前体(LP)细胞。组织培养条件为 是为了诱导这些细胞分化成三维的， 器官类型组织。鉴定表明，该组织复制了许多 原发肿瘤的组织学、超微结构和蛋白表达特性 组织。初步实验证明了该组织模型在 预测ECV刺激性和艾滋病毒感染/传播研究。最后， 对生产这种组织所涉及的成本的经济评估表明 ECV组织产品似乎在商业上是可行的。第二阶段 工作计划将在第一阶段成果的基础上扩展，以进一步发展ECV 组织模型。将优化培养条件，并将组织 彻底刻画的。刺激性研究的方案将最终敲定 而艾滋病毒在组织中的感染和传播机制将是 勾勒出来的。最后，测试艾滋病毒杀微生物剂的方法将是 发展起来的。

DESCRIPTION: (Provided by Applicant) Phase I research adapted primary culture methods to obtain a reliable source of normal ectocervico-vaginal (ECV) epithelial and Langerhans precursor (LP) cells. Tissue culture conditions were developed to induce these cells to differentiate into a three-dimensional, organotypic tissue. Characterization showed that the tissue reproduced many of the histological, ultrastructural, and protein expression properties of native tissue. Initial experiments demonstrated the utility of the tissue model for predicting ECV irritation and for HIV infection/ transmission studies. Finally, an economic assessment of the costs involved in producing such tissues showed that the ECV tissue product appears to be commercially feasible. The Phase II work plan will expand upon the results of Phase I to further develop the ECV tissue model. Culture conditions will be optimized and the tissue will be thoroughly characterized. Protocols for irritation studies will be finalized and the mechanisms of HIV infection and transmission in the tissue will be delineated. Finally, methodology for testing HIV microbicides will be developed.