Tissue procurement and processing for molecular analysis

描述：（申请人提供） 本研究应用程序的目标是为用户提供 合作人体组织网络提供高质量的人体组织样本， 最先进的组织处理服务。采购的组织标本将 新鲜冷冻、化学固定、石蜡包埋和活菌 form.活组织的选择包括冷冻细胞悬液， 机械和酶促分解的固体组织、冷冻细胞 小组织碎片的悬浮液、淋巴细胞的冷冻细胞悬浮液 从淋巴结和活细胞的冷冻细胞悬浮液中解聚 从腹水中获得。除了石蜡包埋，组织学 将提供石蜡包埋或冷冻组织切片。激光辅助 显微解剖将提供给那些需要小但 高度纯化的细胞群。核酸提取（RNA和DNA）将 可用于散装和显微解剖组织样本。组织 微阵列将由正常和肿瘤性人体组织构建， 组织切片将提供给希望进行 使用现场实验方法进行有效的筛选研究。虽然 所有样本将仅通过代码编号（“去识别”）进行识别， 保持高道德研究标准，对于大多数广泛的临床研究， 恶性肿瘤受试者的随访数据可通过 建立肿瘤登记处。 该应用程序将扩展CHTN目前提供的服务， 显微切割、组织微阵列、DNA和RNA 萃取这些技术代表了人类的尖端技术， 组织分析，并被用来进行调查的遗传 以及人类癌症和其他疾病的分子基础。此次扩大 CHTN服务将大大增加其对研究界的价值。

DESCRIPTION: (Provided by Applicant) The objectives of this research application are to provide users of the Cooperative Human Tissue Network with high quality human tissue samples and state of the art tissue processing services. Procured tissue specimens will be available in fresh frozen, chemically-fixed, paraffin-embedded and viable form. Options for viable tissue include frozen cell suspensions from mechanically and enzymatically disaggregated solid tissue, frozen cell suspensions of small tissue fragments, frozen cell suspensions of lymphocytes disaggregated from lymph nodes and frozen cell suspensions of viable cells obtained from ascites fluid. In addition to paraffin embedding, histologic sections of paraffin embedded or frozen tissue will be offered. Laser-assisted microdissection will be available to investigators who require small but highly purified cell populations. Nucleic acid extraction (RNA and DNA) will be offered for both bulk and microdissected tissue samples. Tissue microarrays will be constructed of normal and neoplastic human tissue and histologic sections will be offered to investigators who wish to perform efficient screening studies using in situ experimental methodologies. Although all samples will be identified only by a code number ("de-identified") to maintain high ethical research standards, for most studies extensive clinical follow-up data is available for subjects with malignant neoplasms through an established tumor registry. This application will expand the services that the CHTN currently offers by the inclusion of microdissection, tissue microarray and DNA and RNA extraction. These techniques represent the cutting edge technologies in human tissue analysis, and are being used to perform investigations into the genetic and molecular basis of human cancer and of other diseases. This expansion of CHTN services will greatly increase its value to the research community.