CORE--MASS SPECTROMETRY LABORATORY

质谱学技术，特别是那些需要测量生物基质中稳定(重)核素丰度的技术，已经成为研究各种病理生理条件下的营养和新陈代谢的重要工具。在过去的几年里，CNRU内的营养/代谢研究计划特别推动了这一领域的发展，并将继续致力于更好地定义和表征伴随着各种病理生理状态的营养、燃料和底物代谢的变化。一名兼职经理将与一名训练有素的全职技术人员一起为这一核心设施提供支持。质谱仪的主要功能有四：1.及时准确地测定体液、组织和呼出空气中示踪剂及其衍生物的稳定同位素丰度。2.协助开发血液组织和尿液中化合物的同位素分析新方法，以满足参与中心资助研究计划的研究人员的需要。3.为支持使用陈旧核素作为示踪剂的研究研究而进行的所有分析提供质量控制。4.发挥培训作用，为潜在的新研究者提供机会，了解稳定同位素示踪技术在代谢/营养研究中的应用。

Mass spectrometric techniques, particularly those requiring the measurement of stable (heavy) nuclide enrichment in biological matrices have become essential tools in the study of nutrition and metabolism under various pathophysiological conditions. The nutrition/metabolic research program within the CNRU has given particular impetus to this area over the past years and it continues to be directed towards a better definition and characterization of the changes in nutrient, fuel and substrate metabolism that accompany various pathophysiological states. A part-time manager will support this core facility together with a well trained full-time technician. The principal functions of the mass spectrometry facility are four: 1. To provide timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. 2. To assist in development of new methods for isotope analyses of compounds in blood tissues, and urine, as required to meet the needs of the investigators who are participating in the Center Grant research program. 3. To provide quality control for all analysis conducted in support of the research studies that involve use of stale nuclides as tracers. 4. To serve a training function and provide potential new investigators with an opportunity to learn about the application of stable isotope-tracer techniques in metabolic/nutritional research.