Survival of Microsporidia After Exposure to Disinfectants and Environmental Condi

微孢子虫是一种普遍存在的寄生虫，可引起 对昆虫、鱼和哺乳动物的感染。最近的微孢子虫 已经被证明可以感染人类。这些生物体会导致 眼科和胃肠道感染，主要发生在 获得性免疫缺陷综合征。人类的几个属 病原体是在细胞培养中培养出来的。目前，有以下几种 没有关于人类病原体能力的数据 肠型脑孢子虫和海伦型脑孢子虫的生存 在各种环境条件下。此外，目前还没有报道称 关于消毒剂对这两种细菌孢子的影响 种。微孢子虫孢子暴露后的存活 氯、酒精和季铵盐等消毒剂 化合物和环境条件，如高架 将对温度和干燥进行研究。培育 使用不同细胞系的壳瓶系统中的微孢子虫有 进行了研究，微孢子虫似乎在这两个地方复制 成纤维细胞和上皮细胞系。染色方法正在进行中 检测感染细胞培养物中微孢子虫包涵体的评价 暴露在消毒剂和环境条件下后。一个 由Theodore Nash博士(NIAID， LPD)已经取代了吉姆萨染色来检测受感染的细胞。 尽管单抗可以检测到感染的细胞 更简单的是，化验仍然既繁琐又乏味。为了努力 替代感染细胞培养的染色，一种定量的方法 微孢子虫DNA的研究已经取得进展。这种化验方法利用 聚合酶链式反应和Eu标记的DNA探针 是半定量的。

Microsporidia are ubiquitous parasites causing infections in insects, fish, and mammals. Recently microsporidia have been demonstrated to infect humans. These organisms cause ophthalmic and gastrointestinal infections, primarily in patients with acquired immune deficiency syndrome. Several genera of human pathogens have been cultivated in cell culture. Currently, there are no data regarding the ability of the human pathogens Encephalitozoon intestinalis and Encephalitozoon hellem to survive under various environmental conditions. Also, there are no reports regarding the effects of disinfectants on spores of these two species.The survival of microsporidial spores after exposure to disinfectants such as chlorine, alcohol, and quaternary ammonium compounds, and environmental conditions such as elevated temperature and dessication will be studied. Cultivation of microsporidia in the shell vial system using various cell lines has been investigated and microsporidia appear to replicate in both fibroblast and epithelial cell lines. Staining methods are being evaluated to detect microsporidial inclusions in infected cell culture after exposure to disinfectants and environmental conditions. A monoclonal antibody produced by Dr. Theodore Nash (NIAID, LPD) has replaced giemsa staining for detection of infected cells. Although the monoclonal antibody makes detection of infected cells easier, the assay is still cumbersome and tedious. In an effort to replace staining of infected cell cultures, a method for quantitation of microsporidial DNA has been developed. This assay utilizes polymerase chain reaction and a europium-labeled DNA probe and is semi-quantitative.