ROLE OF ADHESION MOLECULES IN MAST CELL AND BASOPHILS FUNCTION

炎症反应的特征是 损伤部位细胞外基质蛋白的积聚。桅杆 细胞和嗜碱性粒细胞聚集在这样的炎症部位，发挥着 通过释放一系列调解人来发挥作用。嗜碱性粒细胞和肥大细胞 有参与结合的表面黏附受体 这些细胞转移到其他细胞或细胞外基质。这种整合素 细胞与纤维连接蛋白介导的黏附刺激细胞扩散 并产生细胞内信号。在此之前我们观察到 RBL-2H3细胞通过整合素受体与涂层表面结合 纤维连接蛋白；这会导致细胞骨架、细胞 颗粒向周围的扩散和重新分布 这些细胞。这些变化导致酪氨酸磷酸化。 几种蛋白质，包括粘着斑激酶pp125FAK (FAK)和细胞分泌增强。FAK是一个广泛的 表达的蛋白酪氨酸激酶与整合素和 几种蛋白质到细胞接触的胞内位置 细胞外基质。FAK也会变成酪氨酸磷酸化 对包括FceRI在内的各种受体的激活做出反应 和T细胞上的抗原受体。在今年的研究中，我们 继续探索细胞内信号的相互作用 由黏附和免疫受体激活产生。

The inflammatory response is characterized by the accumulation of extracellular matrix proteins at sites of injury. Mast cells and basophils accumulate at such inflammatory sites and play a role by releasing an array of mediators. Basophils and mast cells have surface adhesion receptors that are involved in the binding of these cells to other cells or to the extracellular matrix. Such integrin mediated adherence of cells to fibronectin stimulates cell spreading and generates intracellular signals. Previously we observed that the RBL-2H3 cells bind through integrin receptors to surfaces coated with fibronectin; this results in changes in the cytoskeleton, cell spreading and a redistribution of the granules to the periphery of the cells. These changes result in the tyrosine phosphorylation of several proteins including the focal adhesion kinase, pp125FAK (FAK) and in enhanced secretion by the cells. FAK is a widely expressed protein tyrosine kinase that localizes with integrins and several proteins to intracellular sites where cells are in contact with the extracellular matrix. FAK also becomes tyrosine phosphorylated in response to the activation of various receptors including FceRI and antigen receptors on T-cells. In studies during this year we have continued exploring the interaction of intracellular signals generated by adhesion and immune receptor activation.