TIME RESOLVED SMALL ANGLE XRAY SCATTERING OF REFOLD OF SPERM WHALE APOMYOGLOBIN

我们建议进行停流时间分辨小角x射线。 抹香鲸脱脂蛋白在不同温度下复性的散射研究 在最近的折叠研究中使用的相同条件 停流圆二色谱和脉冲标记核磁共振技术。我们 想要获得更多关于大小和时间尺度的信息 在地球表面形成了一种动态的熔球中间体 载脂蛋白肌红蛋白重折叠途径。这些项目提供的见解 实验将有助于进一步解释核磁共振结果，并应该 使我们对折叠机制有了更清晰的了解 尤其是去肌红蛋白和一般蛋白质。

We propose to perform stopped-flow time-resolved small-angle x-ray scattering studies of the refolding of sperm whale apomyoglobin under the same conditions used in a recent folding study employing stopped-flow circular dichroism and pulse labeling NMR techniques. We want to obtain further information regarding the size and time-scale of formation of a kinetic molten globule intermediate on the apolmyoglobin refolding pathway. The insights provided by these experiments will help to further interpret the NMR results, and should lead to a clearer understanding of the folding mechanism of apomyoglobin in particular and of proteins in general.