Peripheral Blood Monocytes In Myobacterial Infections

单核细胞的特征是CD40、CD80、CD86、CD120b和γ干扰素受体α链的表达水平。这些研究已经确定了两例γ干扰素受体α链缺乏的患者，并且正在积极检查这种缺陷的分子水平。此外，干扰素γ受体β链缺乏症也在分子水平上被表征。作为这些研究的结果，一种用于评估STAT蛋白磷酸化的细胞内流式细胞术方法已经被开发出来，最初用于STAT1，最近用于STAT4。细胞表面受体与细胞因子反应水平的功能状态的结合正在应用于新患者，也被用于评估骨髓移植后患者。

Monocytes are being characterized for the expression levels of CD40, CD80, CD86, CD120b, and the gamma interferon receptor alpha chain. These studies have identified two patients with gamma interferon receptor alpha chain deficiency, and the molecular level of this defect is being actively examined. In addition, interferon gamma receptor beta chain deficiency has also been characterized in one patient at the molecular level. As a consequence of these studies, an intracellular flow cytometric method for evaluating STAT protein phosphorylation has been developed and validated initially for STAT1 and more recently for STAT4. The combination of cell surface receptor and functional status at the level of cytokine response is being applied to new patients and is also being used to evaluate patients after bone marrow transplantation.