Acquisition of a Nanoflow Ion Trap Mass Spectrometer

描述(由申请人提供)：这项提案要求一个Thermo Finnigan ProteomeX Decap-51000综合工作站，一个配备LC泵的离子陷阱质谱仪，一个10端口开关阀，用于多维层析的强阳离子和反相柱，以及一个纳米喷雾探针。该仪器将满足亚利桑那大学一些生物科学研究人员的需求。仪器的主要用户及其应用是(1)Samuel Ward，分子和细胞生物学，研究线虫细胞分化过程中的信号通路，基因与人类阿尔茨海默氏症和肌营养不良相关的疾病基因同源；(2)Brian Larkins，植物科学，农业与生命科学学院，鉴定和分析改善玉米蛋白质营养质量所需的蛋白质；(3)M.Halonen/D.Vercell/F.Martinez/M.Cusanovich，呼吸科学中心，结合免疫球蛋白G4种系启动子、IL-13启动子和CD 14启动子中调节元件的转录因子；哮喘的细胞和分子机制(4)伊丽莎白·维尔林，分子和细胞生物学，分子伴侣功能；细胞质细胞器和热休克蛋白的表达和功能，所研究的通路对正常细胞功能至关重要；(5)Carol Dieckmann，生物化学，鉴定衣藻眼斑主要多肽基因的突变；(6)Thomas Baldwin，生物化学，脉冲烷化MS，研究细菌荧光素酶中的蛋白质折叠；(7)Vicki Wysocki，化学，多肽解离的机理和能量学，这项工作可直接适用于生物体中蛋白质的鉴定。现代蛋白质研究离不开质谱学。使用带有纳米探针的专用微流控液质谱计来表征无法使用当前质谱学设备仪器进行分析的样品，对于这些项目的最大生产率和成功至关重要。该大学对该项目作出了坚定的承诺，包括翻修位于生物科学的一个新的“分支”质谱学实验室的空间，雇用一名全职博士生物质谱学专家(每年约60,000美元)，为一名蛋白质组学主任(约80,000美元/年)和一名技术员(约35,000美元/年)提供资金，以帮助制备样品，并提供75,000美元的费用分摊。

DESCRIPTION (provided by applicant): This proposal requests a Thermo Finnigan ProteomeX DECAP-51000 Integrated Workstation, an ion trap mass spectrometer equipped with LC pumps, a 10 port switching valve, strong cation and reversed phase columns for multidimensional chromatography, and a nanospray probe. This instrument will serve the needs of a number of University of Arizona bioscience researchers. The major users and their applications for the instrumentation are (1) Samuel Ward, Molecular and Cellular Biology, Study of signaling pathways during cell differentiation in the Nematode C.elegans, genes are homologeous to human disease genes linked to Alzheimer's and muscular dystrophy; (2) Brian Larkins, Plant Sciences, College of Agriculture and Life Sciences, Identificationand Analysis of Proteins Required for Improved Maize Protein Nutritional Quality; (3) M. Halonen/D. Vercelli/F. Martinez/M. Cusanovich, Center for Respiratory Sciences, Transcription Factors that Bind Regulatory Elements in the Immunoglobulin G4 Germline Promoter, the IL-13 Promoter, and the CD 14 Promoter; Cellular and Molecular Mechanisms of Asthma (4) Elizabeth Vierling, Molecular and Cellular Biology, Molecular chaperone function; expression and function of cytoplasmic organelle and heat shock proteins, the pathways studied are critical to normal cell function; (5) Carol Dieckmann, Biochemistry, Identification of Mutations in Genes Coding for Major Polypeptides in the Chlamydomonas Eyespot;, (6) Thomas Baldwin, Biochemistry, Pulsed Alkylation MS to Investigate Protein Folding in Bacterial Luciferase; (7) Vicki Wysocki, Chemistry, Mechanisms and Energetics of Peptide Dissociation, this work is directly applicable to the identification of proteins from biological organisms. Modern protein research cannot be accomplished without mass spectrometry. The access to a dedicated microflow LC-mass spectrometer with a nanospray probe to characterize samples that are not amenable to analysis with the current mass spectrometry facility instruments is critical to the maximum productivity and success of these projects. The University has made a strong commitment to the project by by renovating space for a new "branch" mass spectrometry laboratory that is located in Biosciences, by hiring a full time Ph.D. biological mass spectrometry specialist (about $60,000 per year), by providing funds for a Director of Proteomics (about $80,000/year) and a technician (about $35,000/year) to help with sample preparation, and by providing cost sharing in the amount of $75,000.