Core--Proteomics Facility

Siteman癌症中心（SCC）蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多重蛋白质组学分析。这些方法包括使用多种荧光染料进行二维差异凝胶电泳以定量和检测功能基团，使用串联质谱法（LC-MS/MS和MALDI-TOF/-TOF）进行蛋白质鉴定，以及使用差异稳定同位素标记的多维液相色谱法。SCC蛋白质组学核心正在与SCC生物信息学核心（Jeffrey Milbrandt，核心主任）和多重基因分析核心（Mark沃森，核心主任）合作，建立实验室信息管理系统（LIMS）和关系数据库，使SCC研究人员能够访问和查询临床、蛋白质组学和 mRNA阵列数据通过一个单一的医学信息学门户。

The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.