System for Multiplex Real-Time Analysis of Nucleic Acids

描述(由申请人提供)： 这一阶段项目的目标是测试生物芯片成像系统的可行性，该系统将改变阵列的温度，并在聚合酶链式反应和探针熔融过程中进行实时成像和定量。一种专门的离散水合物微阵列！用于阵列聚合酶链式反应扩增的隔室将用原型仪器进行测试。预计该技术将适用于重要的应用，如基因表达、多态性评分和微生物检测和分型。样品和试剂的消耗将降至最低，因为可以在小体积内实现显著的多重因子。该项目的预期结果是指定一种可用于研究或诊断的多功能仪器，并可将其带入开发阶段。

DESCRIPTION (provided by applicant): The objective of this Phase I project is to test the feasibility of biochip imaging system which will vary the temperature of the arrays and perform real-time imaging and quantification during PCR and probe meltoff. A specialized microarray of discrete hydroge! Compartments for array-based PCR amplification will be tested with the prototype instrument. The technology is expected to be appropriate for significant applications such as gene expression, polymorphism scoring and microbial detection and typing. Consumption of sample and reagents will be minimized, because significant multiplex factors can be realized in small volumes. The expected outcome of this project is the specification of a versatile instrument for research or diagnostics that could be brought into the development phase.