CORE--MICROSCOPIC IMAGING

组织培养/杂交瘤：核心的六名研究人员中有四人拥有组织培养设施 他们拥有自己的实验室，为了更有效地运营和集中，他们希望将这些实验室整合到一个核心组织培养设施中。该核心将提供永生化和连续细胞系的维护；维持冷冻保存的细胞系库存；建立原代角膜和视网膜细胞系；维护人葡萄膜黑色素瘤细胞系的肿瘤库并启动新的细胞系；培养杂交瘤细胞；纯化和浓缩单抗；筛选支原体和内毒素污染的细胞系和杂交瘤细胞；使用SV40大T抗原或其他制剂生产永生细胞系。这些设施将被安置的空间被很好地定义，并且在每个非感应者都很容易到达的地方。六名调查员中的五名将是这一核心的广泛用户。(由于某种原因，詹姆斯·麦卡利没有列在这一部分，他的名字也没有出现在第467页的用户表中)。这是一个经过深思熟虑的模块，它将提高该计划参与者的工作效率。其组织中唯一的弱点是尼德科恩博士，这笔基础设施拨款的主要研究员 还担任模块总监。令人惊讶的是，另一名调查员没有被指定为单元主任，因为剩下的五名调查员中有四人被列为广泛用户。

Tissue Culture/Hybridoma: Four of the six investigators in the core have tissue culture facilities in their own labs, and for more efficient operation and centralization they wish to consolidate these into a core tissue culture facility. This core will provide maintenance of immortalized and continuous cell lines; maintain cryo-preserved cell line stocks; establish primary corneal and retinal cell lines; maintain tumor bank of human uveal melanoma cell lines and start new ones; cultivate hybridomas; purify and concentrate monoclonal antibodies; screen cell lines and hybridomas for mycoplasma and endotoxin contamination; produce immortal cell lines using SV40 large T-antigen or other agents. The space where these facilities will be housed is well defined and is within easy access of each invistigator. Five of the six investigators will be extensive users of this core. (For some reason James McCully was not listed in this section, and his name does not appear in the Table of users on page 467). This is a well thought out module, which will enhance the productivity of the participants in this program. The only weakness in its organization is that Dr. Niederkorn, the principal investigator of this infractructure grant also serves as Module Director. It is surprising that one of the other investigators was not designated as module director since four of the five remaining investigators are listed as extensive users.