Microscopy and Digital Imaging

资源/服务核心：显微镜和数字成像 摘要： 显微镜和数字成像模块将提供设备、基础设施支持、专门知识和 为视觉科学家提供技术援助，以便能够对细胞和组织进行详细的定量成像。 这一最先进的成像设备包括几件高端图像采集和分析 硬件，以及支持和加强正在进行的研究和探索新研究所需的专业知识 视觉科学家提出的问题。设施运营将包括：1)维护和维护 设备，2)管理设备的使用，3)教育调查人员关于 设备，4)就视觉研究项目的成像实验设计向调查人员提供建议，5) 提供有关设备正确使用的培训，以及6)帮助研究人员收集、处理和 分析当前和未来应用的高质量显微镜数据。 显微成像设施内提供的具体服务和技术包括： A)细胞和组织加工、标记和免疫细胞化学； B)光学和荧光显微镜， C)激光扫描共聚焦显微镜， D)多光子显微镜和时间推移成像， E)活体共聚焦显微镜，以及 F)形态定量和图像分析。 总体而言，显微镜和数字成像模块将支持一系列成像技术 参与调查人员，并便利轻松获得设备、培训和技术支持 扩大实验能力，开拓新的研究方向，产生更具性价比、更及时的-- 高效和严谨的成像研究。另一个目标是使用这些切割来促进协作研究 UTSW的视觉科学家与该地区其他机构之间的边缘成像技术。

Resource/Service Core: Microscopy and Digital Imaging Abstract: The Microscopy and Digital Imaging Module will provide equipment, infra-structural support, expertise and technical assistance to vision scientists in order to enable detailed, quantitative imaging of cells and tissues. This state-of-the-art imaging facility includes several pieces of high-end image acquisition and analysis hardware, and the expertise needed to support and enhance ongoing research and explore new research questions posed by vision scientists. Facility operations will include: 1) maintaining and servicing the equipment, 2) managing usage of the equipment, 3) educating investigators on the capabilities of the equipment, 4) advising investigators on the design of imaging experiments for vision research projects, 5) providing training on the proper usage of the equipment, and 6) helping researchers collect, process and analyze high quality microscopy data for both current and future applications. The specific services and technologies provided within the Microscopic Imaging Facility are: a) cell and tissue processing, labeling and immunocytochemistry, b) light and fluorescent microscopy, c) laser scanning confocal microscopy, d) multiphoton microscopy and time-lapse imaging, e) in vivo confocal microscopy, and f) quantitative morphometry and image analysis. Overall, the Microscopy and Digital Imaging Module will support a range of imaging technologies for participating investigators, and facilitate easy access to equipment, training and technical support that will expand experimental capabilities, open up new research directions, and result in more cost-effective, time- efficient and rigorous imaging studies. An additional goal is to facilitate collaborative studies using these cutting edge imaging techniques between vision scientists at UTSW and other institutions in the region.