Microscopy and Digital Imaging

显微镜和数字成像

• 批准号: 10438811
• 负责人: W MATTHEW PETROLL
• 金额: $ 22.31万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438811
• 关键词: Address; Cells; Confocal Microscopy; Contract Services; Core Grant; Cytoskeleton; Data; Equipment; Extracellular Matrix; Fees; Funding; Future; Goals; Image; Image Analysis; Imaging Techniques; Imaging technology; Institution; Label; Laser Scanning Confocal Microscopy; Light; Maintenance; Microscopy; Process; Records; Research; Research Personnel; Research Project Grants; Resources; Scientist; Services; Structure; Technology; Time; Tissues; Training; Vision; Vision research; Visualization; cellular imaging; cost; cost effective; design; digital; digital imaging; equipment training; experimental study; image processing; image visualization; imaging facilities; imaging software; imaging study; immunocytochemistry; investigator training; microscopic imaging; morphometry; multiphoton microscopy; operation; quantitative imaging; reflectance confocal microscopy; tissue processing

Resource/Service Core: Microscopy and Digital Imaging Abstract: The Microscopy and Digital Imaging Module will provide equipment, infra-structural support, expertise and technical assistance to vision scientists in order to enable detailed, quantitative imaging of cells and tissues. This state-of-the-art imaging facility includes several pieces of high-end image acquisition and analysis hardware, and the expertise needed to support and enhance ongoing research and explore new research questions posed by vision scientists. Facility operations will include: 1) maintaining and servicing the equipment, 2) managing usage of the equipment, 3) educating investigators on the capabilities of the equipment, 4) advising investigators on the design of imaging experiments for vision research projects, 5) providing training on the proper usage of the equipment, and 6) helping researchers collect, process and analyze high quality microscopy data for both current and future applications. The specific services and technologies provided within the Microscopic Imaging Facility are: a) cell and tissue processing, labeling and immunocytochemistry, b) light and fluorescent microscopy, c) laser scanning confocal microscopy, d) multiphoton microscopy and time-lapse imaging, e) in vivo confocal microscopy, and f) quantitative morphometry and image analysis. Overall, the Microscopy and Digital Imaging Module will support a range of imaging technologies for participating investigators, and facilitate easy access to equipment, training and technical support that will expand experimental capabilities, open up new research directions, and result in more cost-effective, time- efficient and rigorous imaging studies. An additional goal is to facilitate collaborative studies using these cutting edge imaging techniques between vision scientists at UTSW and other institutions in the region.

资源/服务核心：显微镜和数字成像 摘要： 显微镜和数字成像模块将提供设备、基础设施支持、专业知识和 为视觉科学家提供技术援助，以便能够对细胞和组织进行详细的定量成像。 这种最先进的成像设备包括几个高端图像采集和分析 硬件，以及支持和加强正在进行的研究和探索新的研究所需的专业知识 视觉科学家提出的问题。设施运作将包括：1）维护和维修 设备，2）管理设备的使用，3）教育调查人员的能力， 设备，4）为视觉研究项目的成像实验设计提供咨询，5） 提供正确使用设备的培训，以及6）帮助研究人员收集，处理和 为当前和未来的应用分析高质量的显微镜数据。 显微成像设施提供的具体服务和技术包括： a）细胞和组织处理、标记和免疫细胞化学， B）光学和荧光显微术， c）激光扫描共聚焦显微镜， d）多光子显微术和延时成像， e）体内共焦显微术，和 f）定量形态测量和图像分析。 总的来说，显微镜和数字成像模块将支持一系列成像技术， 参与的调查人员，并方便获得设备，培训和技术支持， 扩大实验能力，开辟新的研究方向，并导致更具成本效益，时间- 有效和严格的成像研究。另一个目标是促进合作研究使用这些切割 边缘成像技术之间的视觉科学家在UTSW和该地区的其他机构。