CORE--CELL CULTURE

细胞培养技术在现代生物医学研究中发挥着至关重要的作用，特别是在 癌症研究。细胞培养设施(CCF)作为定制的付费服务设施 提供专业知识、技术支持、试剂、设备、液氮存储和/或 培训以提高在fccc中使用哺乳动物细胞培养的有效性和效率 实验室。该中心提供以下方面的技术援助和咨询服务：组织培养技术、中央液氮库、支原体筛查服务、定制和标准培养液和补充剂的制备、胎牛血清的性能测试、细胞繁殖，以及小鼠胚胎干细胞培养的技术支持和用品。CCF的主要努力包括实施具有技术挑战性的技术，例如从患者或动物组织样本建立原代细胞培养，以及产生基因“敲除”或“敲除”ES细胞。2003年，该机制为52名调查人员提供了同行审查的资金，并为来自所有三个司和11个研究方案的联合国气候变化框架委员会65个研究实验室中的56个提供了服务。同行评审、资助的研究占CCF使用量的92.5%以上。该设施在教师用户中享有很高的声誉，并在上次CCSG审查中获得了“优秀到优秀”的评级。在过去的五年中，对CCF服务的需求大幅增加，建立的原代细胞系增加了225%，培养液、无菌试剂和血清的分布增加了54%-117%。重要的是，该机制为fccc社区提供了宝贵的现场专业知识，帮助制定了一系列细胞培养方案，咨询了实验设计和评估，培训了细胞培养方法，并以具有成本效益的方式提供了适应性援助。该基金继续提供创新服务，包括最近建立了“核导入”服务，以有效地将细胞系转入细胞系，并生产用于“三维”细胞培养的细胞外基质。因此，CCF为fccc调查人员提供了现成的专业知识、人力资源，并为文化用品和专门服务提供了方便和具有成本效益的资源。

Cell culture technology plays an essential role in modern biomedical research, especially in cancer research. The Cell Culture Facility (CCF) serves as a customized pay-for-service Facility that provides expertise, technical support, reagents, equipment, liquid nitrogen storage, and/or training to increase the effectiveness and efficiency of using mammalian cell culture in FCCC laboratories. This Facility provides technical assistance and consultation services in tissue culture techniques, centralized liquid nitrogen banking, mycoplasma screening services, the preparation of custom and standard culture media and supplements, performance testing of fetal bovine serum, cell propagation, and technical support and supplies for mouse embryonic stem (ES) cell culture. Major efforts by the CCF include carrying out technically-challenging techniques, such as the establishment of primary cell cultures from patient or animal tissue samples and the generation of gene "knockout" or "knockin" ES cells. In 2003, the Facility served 52 investigators with peer-reviewed funding and a total of 56 of the 65 research laboratories at FCCC from all three Divisions and 11 Research Programs. Peer-reviewed, funded research accounted for more than 92.5% of CCF usage. The Facility is highly-regarded among faculty users, and received an "Outstanding to Excellent" rating at the last CCSG review. In the past five years, the demand for CCF services has increased substantially, with 225% more primary cell lines established and 54-117% increases in the distribution of media, sterile reagents, and serum. Importantly, the Facility supplies the FCCC community with valuable on-site expertise for a host of established cell culture protocols, consultation in the design and evaluation of experiments, training in cell culture methodologies, and adaptable assistance delivered in a cost-effective manner. The Facility continues to provide innovative services, including the recent establishment of "nucleofection" services to efficiently transfect cell lines and the production of extracellular matrices for "three-dimensional" cell culture. Thus, the CCF serves FCCC investigators with a ready source of expertise, manpower, and a convenient and cost-effective resource for culture supplies and specialized services.