CORE--GENETIC MONITORING

这一快速增长的遗传监测设施由20个同行审查、资助的 来自8个研究项目的研究人员在36个研究方案中使用动物模型。二零零三年， 利用该基金的研究项目中有97.7%得到了同行审查资金的支持。 良好的基因监测已经成为狐狸大通实验室动物需求的关键 作为今天生物医学研究中使用的动物，癌症中心(Fccc)的研究社区不仅必须没有疾病，而且还必须根据它们的基因构成对其进行良好的定义。目前，我们监测了位于9条不同染色体上的11个不同的基因座。我们目前为fccc研究人员维护了170个小鼠品系，并继续平均每月增加一个新的转基因品系。在过去的一年里，该设施进行了5856次基因图谱测试，比1999年的1800次增加了225%。遗传监测服务的服务时间也大幅增加，从1999年的342小时增加到2003年的821小时，增幅为140%。繁育群体的日常运作由在遗传管理和记录保存方面训练有素和经验丰富的人员处理。由2004年3月加入的遗传学家顾问Bruce Elder博士定期提供审查、建议和指导，他每季度访问并审查所有啮齿动物群体的报告和活动，所有繁殖、生产和遗传监测记录都已电脑化。这些计算机化的记录使我们能够密切监测育种性能、系谱系以及各种自交系和品系的利用情况。监测独特的免疫缺陷和转基因小鼠的基因组和维持繁殖群体的能力加强了免疫生物学计划成员以及细胞和发育生物学(Burch)、Mintz(肿瘤细胞生物学)、人类遗传学(Testa，计划负责人)、发展治疗(Kruh，Smith，Weiner，计划负责人)和癌症预防和控制(Clapper)计划成员之间的互动。遗传监测服务已经成为动物研究的关键部分，预计未来对服务的需求将继续以每年18%的速度增长。我们建议增加一个FTE，以支持设施活动的这一增加。

This rapidly growing Genetic Monitoring Facility is used by 20 peer-reviewed, funded investigators from 8 Research Programs using animal models in 36 research protocols. In 2003, 97.7% of the research projects utilizing this Facility were supported by peer-reviewed funding. Good genetic monitoring has become critical to the laboratory animal needs of the Fox Chase Cancer Center (FCCC) research community, as animals used in today's biomedical research must not only be free of disease, but also be well defined in terms of their genetic makeup. At present, we monitor 11 different loci located on 9 different chromosomes. We are currently maintaining 170 lines of mice for FCCC investigators and continue to add an average of one new transgenic line per month. During the past year, the Facility has performed 5,856 genetic profile tests, a 225% increase from the 1,800 performed in 1999. Service hours for genetic monitoring services have also increased dramatically, up 140% from 342 in 1999 to 821 in 2003. The daily operation of breed ng colonies is handled by personnel trained and experienced in genetic management and record-keeping. Regular review, advice and guidance is provided by Bruce Elder, Ph.D., a consultant geneticist added in March 2004 who makes quarterly visits and reviews reports and activities in all rodent colonies, and all of the breeding, production and genetic monitoring records have been computerized. These computerized records enable us to closely monitor breeding performance, pedigree lines, and utilization of the various inbred lines and strains. The ability to monitor the genome, and maintain the breeding colonies, of unique immunodeficient and transgenic mice enhances the interaction among Members of the Immunobiology Program as well as Members of the Cellular and Developmental Biology (Burch), Mintz (Tumor Cell Biology), Human Genetics (Testa, Program Leader), Developmental Therapeutics (Kruh, Smith, Weiner, Program Leader), and Cancer Prevention and Control (Clapper) Programs. Genetic Monitoring services have become a critical part of research with animals, and the need for services is expected to continue to increase 18% per year in the future. We propose adding one FTE to support this increase in Facility activities.