CORE--CELL IMAGING

细胞成像设备为35名同行评审人员提供高分辨率成像服务 来自福克斯·蔡斯癌症中心所有三个部门的10个研究项目的研究人员。 2003年，99%(99%)的使用是为了支持同行审查、资助的调查人员。成像软件和硬件的最新技术进步使高分辨率显微镜成为生物医学研究人员必不可少的不可或缺的技术。由于技术的快速进步，这一共享设施为研究人员提供了访问最先进的成像设备的途径。这一设施不仅为现有调查人员提供基本服务，而且也是未来调查人员的有效招聘工具。该设施的使用时间从1999年至2001年的平均800小时，分别增加到2002年和2003年的2000小时和1100小时。这一急剧增长反映了新的和现有的调查人员对高分辨率成像的需求增加。为了满足用户的需要，该设施现在对所有受过培训的用户全天候开放。自2004年5月实施这些变化以来，该设施的每月平均使用量稳步增加，从每周约40小时增加到每周约80小时。该设施支持三大类显微技术：1)通过共聚焦或传统的免疫荧光显微镜在细胞内定位单个或多个蛋白质(最多三个)，2)执行依赖于显微注射和视频显微镜的单细胞实验，3)荧光标记蛋白质的实时可视化。支持这些服务的设备包括一台新升级的Biorad Radiance 2000激光扫描共焦显微镜(LSCM)，以及两台升级的尼康倒置显微镜，配备了荧光光学元件和高灵敏度CCD摄像机。利用Metamorph软件进行图像采集和处理。 设施人员的作用是向用户提供技术援助，使他们成为 精通图像采集和处理。该设施还将协助用户在 影像实验的设计与实现。该设施维护设备，并 负责向调查人员整合和介绍新技术。

The Cell Imaging Facility provides high resolution imaging services to 35 peer-reviewed investigators from 10 research Programs in all three Divisions at Fox Chase Cancer Center. Ninety-nine percent (99%) of the use in 2003 was in support of peer-reviewed, funded investigators. Recent technological advances in imaging software and hardware has made high resolution microscopy an essential and integral technology for biomedical researchers. Because of rapid technological advances, this shared Facility provides investigators access to state-of-the-art imaging equipment. This Facility not only provides essential services to existing investigators, but is also an effective recruiting tool for prospective investigators. Usage of this Facility increased from an average of 800 hours for years 1999 to 2001, to 2000 and 1,100 hours for years 2002 and 2003, respectively. The dramatic increase reflects increased needs of new and existing investigators for high resolution imaging. To accommodate users' needs, the Facility is now open 24/7 to all trained users. Since implementing these changes in May of 2004, the average monthly usage of the Facility has steadily increased from approximately 40 hours per week to approximately 80 hours per week. This Facility supports three broad categories of microscopic techniques: 1) localization of single or multiple proteins (up to three) within a cell by confocal or conventional immunofluorescence microscopy, 2) perform single cell experiments that rely on microinjection and video microscopy, 3) real-time visualization of fluorescent-tagged proteins. The equipment that supports these services includes a newly upgraded: BioRad Radiance 2000 Laser Scanning Confocal Microscope (LSCM), and two upgraded Nikon inverted microscopes that are equipped with epifluorescence optics and high sensitivity CCD cameras. Image acquisition and processing are conducted with Metamorph software. The role of Facility personnel is to provide technical assistance to users so that they become proficient with both image acquisition and processing. The Facility will also assist users in the design and implementation of imaging experiments. The Facility maintains the equipment and is responsible for incorporating and introducing new technologies to investigators.